Design and Performance of a Real-time RT-PCR Assay For Detection of Influenza C Viruses.

Details

• Alternative Title:
  Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 2025, v. 181: Design and Performance of a Real-time RT-PCR Assay For Detection of Influenza C Viruses.
• Journal Article:
  Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
• Personal Author:
  Shu, Bo ; Davis, William G. ; Liu, Ji ; Thielen, Beth K. ; Bistodeau, Sarah ; Lynch, Brian ; Warnes, Christine M. ; Liddell, Jimma ; Strain, Anna K. ; Christensen, Jaime ; Wong, Phili ; Burnett, Natasha ; Davis, Todd C. ; Kirby, Marie K.
• Description:
  Influenza C virus (ICV) usually causes a mild upper respiratory tract infection in children and those infected are frequently co-infected with other respiratory viruses. However, there have only been a few hundred documented cases of ICV infection in humans as of the end of 2024. To better understand the epidemiology and clinical impact of ICVs, we developed an influenza C real-time RT-PCR (InfC rRT-PCR) assay that targets a highly conserved region of the matrix gene segment of ICVs. The analytical sensitivity evaluation demonstrated that the InfC rRT-PCR assay was highly sensitive, as it was able to detect as few as five RNA copies per PCR reaction and had robust reactivity over a range of viral RNAs from historical and recent ICVs. The analytical specificity evaluation confirmed the assay did not cross-react with any influenza A or B viruses tested, including several animal-origin viruses, or other common non-influenza respiratory viruses. The performance evaluation on clinical specimens demonstrated the assay was highly sensitive and specific for the detection of ICVs.
• Content Notes:
  Author manuscript
• Subjects:
  • Humans
  • Influenza, Human
  • Sensitivity and Specificity
  • Real-Time Polymerase Chain Reaction
  • Gammainfluenzavirus
  • Reverse Transcriptase Polymerase Chain Reaction
  • Molecular Diagnostic Techniques
  • RNA, Viral
  • Child
  • Child, Preschool
  More +
• Keywords:
  • Influenza C virus
  • Diagnostic assay
  • Real-time RT-PCR
• Source:
  Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 2025, v. 181
• DOI:
  https://doi.org/10.1016/j.jcv.2025.105874
• ISSN:
  1386-6532 ; 1873-5967
• Pubmed ID:
  41033150
• Pubmed Central ID:
  PMC12974223
• Document Type:
  Text
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Genre:
  Journal Article
• Pages in Document:
  105874 (16 pdf pages)
• Volume:
  181
• Download URL:
  https://stacks.cdc.gov/view/cdc/261023/cdc_261023_DS1.pdf
• File Type:
  [PDF - 642.71 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:665b8ea69c46d7ff42ac7c1787eea8da061bbe3eacac4166d411e48d8ee145c4315cb998de40b374bdf4531fecfa71495b1364a0e72a94f7314b0a1703b09134