Gene Expression in Tumor and Adjacent Normal Tissues in Lung Adenocarcinoma Subtypes

Details

• Journal Article:
  BMC Cancer
• Personal Author:
  Gorlova, Olga Y. ; Gorlov, Ivan P. ; Ripley, R. Taylor ; Cheng, Chao ; Li, Yafang ; Peng, Bo ; Liu, Yanhong ; Jang, Hee-Jin ; Kang, Sung Wook ; Lee, Claire ; Ranchod, Priyanka ; Burt, Bryan M. ; Lee, Hyun-Sung ; Amos, Christopher I.
• Description:
  Background: Lung adenocarcinoma (LUAD) has several histologically distinct subtypes that differ by a number of clinical features including patient survival. Molecular mechanisms underlying histological and clinical differences between subtypes remain poorly understood. Methods: We conducted a comparative analyses of gene expression in acinar, lepidic, papillary and solid subtypes, as well as mucinous adenocarcinoma. We used a novel, more efficient approach to identify subtype-specific genes. We compared the mean gene expression level separately for tumors and adjacent normal tissue with pure or a highly represented (≥ 75%) subtype of interest to the mean expression in tumors where the subtype of interest was not present. We also performed tumor to adjacent normal tissue comparisons and identified genes differentially expressed between tumor and adjacent normal tissues for each subtype. Results: The number of subtype-specific genes varied from 1 for the acinar to 482 for the papillary subtype. Comparative analysis of gene expression in adjacent normal tissues also identified subtype-specific genes, 38 in total. Gene set enrichment analysis identified oxidative phosphorylation as a biological function associated with papillary, and immune response - with solid subtype. Using data on differential expression between tumor and adjacent normal tissue among the subtype-specific genes and existing evidence for association with lung carcinogenesis, we have identified several candidate subtype-specific driver genes. Conclusion: We identified subtype-specific genes, biological functions, and potential drivers of subtype-specific carcinogenesis for LUAD subtypes. The study showed importance of gene expression in adjacent normal tissue for subtype-specific tumorigenesis.
• Subjects:
  Lung Lung Neoplasms Neoplasms Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Lung Adenocarcinoma; LUAD; Predominant Subtypes; Gene Expression Lung Cancer; Adenocarcinomas; Gene Expression; Genetic Factors; Carcinogenesis; Tumorigenesis;
• Source:
  BMC Cancer 2025 Jul; 25:1169
• ISSN:
  1471-2407
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Pennsylvania ; Texas ; New Mexico ; California ; Philadelphia Region [OSHA] ; Dallas Region [OSHA] ; San Francisco Region [OSHA]
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  9 pdf pages
• Volume:
  25
• NIOSHTIC Number:
  nn:20071482
• Email:
  ivan.gorlov@bcm.edu
• Federal Fiscal Year:
  2025
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20060901
• End Date:
  20260831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d852b8a48d9b4f55af64d7771734af14d5a0578c353ff5240d8f4070ee9d93ad83456ad7abd4362367478894c0e5ce8a5c4228988fd277100059ca5b582d4e44
• Download URL:
  https://stacks.cdc.gov/view/cdc/259080/cdc_259080_DS1.pdf
• File Type:
  [PDF - 1.16 MB ]