Ozone Generation Method Impacts Lung Toxicity and Oxidant Signaling

Details

• Journal Article:
  American Journal of Respiratory Cell and Molecular Biology
• Personal Author:
  Razazan, Atefeh ; Mazumder, Md Habibul Hasan ; Goldsmith, William T. ; Tan, Nina O. ; Ranpara, Anand C. ; Kodali, Vamsi K. ; Velayutham, Murugesan ; Wang, Qiang ; Tighe, Robert M. ; Hussain, Salik
• Description:
  Ozone (O3) is a criteria pollutant that is anticipated to increase over the next decade because of climate-related activity. Varying amounts of nitrogen oxides (NOx) are produced as byproducts during O3 generation from oxygen depending on the method of production, including the source and oxygen purity. A review of the current literature confirms a lack of consistent monitoring and reporting of potential nitrogen species produced with different methods of experimental O3 generation. The lack of consistent monitoring and reporting is potentially a factor that can explain divergence of reported experimental O3 exposure outcomes from different research groups. In the present report, we compare the effects of O3 generation from either a filtered air (FA-ozone) or a pure oxygen (Oxy-ozone) source on NOx generation and measures of O3-induced lung injury. We also consider if this impacts mixed exposures with O3 and ultrafine carbon black (CB) based on if the O3 was generated from a filtered air (FA-ozone-CB) versus a pure oxygen (Oxy-ozone-CB) source. Comparing FA-ozone versus Oxy-ozone, we observed increased lung inflammation and injury in the FA-ozone group. In the FA-ozone-CB group, compared with the Oxy-ozone-CB group, the FA-ozone-CB inhalation exposure resulted in the formation of a greater amount of NOx and induced protein nitrotyrosine in the lungs. Moreover, the FA-ozone-CB group had evidence of eosinophil recruitment not observed in the Oxy-ozone-CB group. Overall, this suggests that the source of oxygen for O3 generation impacts experimental outcomes. Furthermore, measurement and reporting of nitrogen species in O3 exposure should be considered.
• Subjects:
  Environmental Monitoring Inhalation Exposure Lung Nitrogen Oxides Occupational Health Ozone Safety Soot
• Keywords:
  Author Keywords: Ozone; Carbon Black; Inhalation Exposure; Nitrogen Oxides; Inflammation Ozone; Nitrogen Oxides; Air Pollutants; Environmental Monitoring; Toxic Effects; Lung Tissue; Immune Reaction; Inhalation; Carbon Black;
• Source:
  Am J Respir Cell Mol Biol 2026 Jan; 74(1):18-26
• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  West Virginia ; North Carolina ; Philadelphia Region [OSHA] ; Atlanta Region [OSHA]
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  9 pdf pages
• Volume:
  74
• Issue:
  1
• NIOSHTIC Number:
  nn:20071189
• Contact Point Address:
  Salik Hussain, D.V.M., Ph.D., Department of Physiology, Pharmacology & Toxicology Robert C. Byrd Health Sciences Center, HSC-N-3074, School of Medicine, west Virginia University, Morgantown, WV 26506
• Email:
  salik.hussain@hsc.wvu.edu
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6) ; Carbon black (CAS RN 1333-86-4)
• Federal Fiscal Year:
  2026
• Peer Reviewed:
  True
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:98da7e74917baf6080e45379c79da268c67b126ac2063c45ca10b1a38f065f783e1bb2d71da0aa1ef734b51356b92e09522037131adc2487245a97e15630fe03
• Download URL:
  https://stacks.cdc.gov/view/cdc/258669/cdc_258669_DS1.pdf
• File Type:
  [PDF - 1.67 MB ]