Evaluation of Dried Blood Spots with a Multiplex Assay for Measuring Recent HIV-1 Infection

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Curtis, Kelly A. ; Ambrose, Krystin M. ; Kennedy, M. Susan ; Owen, S. Michele
• Description:
  Laboratory-based HIV tests for recent infection (TRIs), which primarily measure a specific serological biomarker(s) that distinguishes recent from long-term HIV infection, have facilitated the estimation of population-based incidence. Dried blood spots (DBS) on filter paper are an attractive sample source for HIV surveillance, given the simplified and cost-effective methods of specimen collection, storage, and shipment. Here, we evaluated the use of DBS in conjunction with an in-house multiplex TRI, the HIV-1-specific Bio-Plex assay, which measures direct antibody binding and avidity to multiple HIV-1 analytes. The assay performance was comparable between matched plasma and DBS samples from HIV-1 infected individuals obtained from diverse sources. The coefficients of variation, comparing the median antibody reactivity for each analyte between plasma and DBS, ranged from 2.78% to 9.40% and the correlation coefficients between the two sample types ranged from 0.89 to 0.97, depending on the analyte. The correlation in antibody reactivity between laboratory and site-prepared DBS for each analyte ranged from 0.87 to 0.98 and from 0.90 to 0.97 between site-prepared DBS and plasma. The correlation in assay measures between plasma and DBS indicate that the sample types can be used interchangeably with the Bio-Plex format, without negatively impacting the misclassification rate of the assay.
• Subjects:
  Antibody Affinity Dried Blood Spot Testing Epidemiological Monitoring HIV-1 HIV Infections Homosexuality, Male Humans Male Plasma RNA, Viral / Blood Specimen Handling Viral Load

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• Source:
  PLoS One. 2014; 9(9).
• Document Type:
  Journal Article
• Volume:
  9
• Issue:
  9
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f4c21e4f5c08b56ef510485837b5ec0662444dd5c72de1e86f88b38898faaa7f
• Download URL:
  https://stacks.cdc.gov/view/cdc/25404/cdc_25404_DS1.pdf
• File Type:
  [PDF - 276.67 KB ]