Seasonal and areal variability in PM 2.5 poses differential degranulation and pro-inflammatory effects on RBL-2H3 cells.

Details

• Personal Author:
  Chen J ; Hong Y ; Huang W ; Luo X-S ; Pang Y ; Pinkerton KE ; Tang M ; Wu L
• Description:
  PM2.5 pollution is a widespread environmental and health problem, particularly in China. Besides leading to well-known diseases in the respiratory system, PM2.5 can also alter immune function to induce or aggravate allergic diseases. To determine whether there are temporal and spatial differences in the allergic responses to PM2.5, monthly samples were collected from four regions (urban, industrial, suburban, and rural areas) through a whole year in Nanjing city, China. Inorganic chemical components (metals and water-soluble ions) of PM2.5 were analyzed, and the rat basophil cells (RBL-2H3) exposed to PM2.5 were assessed through quantitative measures of degranulation (beta-hex and histamine) and pro-inflammation cytokine (IL-4 and TNF-a) expression. The highest levels of beta-hex were measured in winter and spring PM2.5 from urban and industrial areas, or autumn PM2.5 from suburban and rural areas. With respect to histamine, autumn PM2.5 samples were most potent irrespective of the location. Autumn and winter PM2.5 induced higher levels of IL-4 than spring and summer samples. However, spring and autumn PM2.5 caused higher levels of TNF-a. The concentrations of water-soluble ions (NH4+, K+ and Cl-), as well as heavy metals (Pb and Cr), were directly and statistically correlated to the inflammation observed in vitro. In general, the differences between regional and seasonal PM2.5 in stimulating cell degranulation may depend on endotoxin and airborne allergen content of PM2.5. The heavy metals and water-soluble ions in PM2.5 were mostly anthropogenic, which increased the particles' mass-based cellular inflammatory potential, therefore, their health risks, e.g. from vehicular exhaust, coal, and biomass combustion, cannot be ignored. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollution Environmental Exposure Hypersensitivity Immune System Occupational Health Particulate Matter Safety
• Keywords:
  Author Keywords: Atmospheric Fine Particulate Pollution; Health Effects; Allergy; Inflammation; Temporal And Spatial Changes; Urban Functional Areas Particulates; Air Pollution; Aerosol Particles; Allergies; Allergic Disorders; Immune Reaction; Seasonal Factors; Histamines; Environmental Exposure; Cellular Reactions;
• ISSN:
  0045-6535
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  279
• NIOSHTIC Number:
  nn:20065603
• Citation:
  Chemosphere 2019 Sep; 279:130919
• Contact Point Address:
  Xiao-San Luo, Nanjing University of Information Science & Technology, No.219, Ningliu Road, Pukou District, Nanjing City, Jiangsu Province, China
• Email:
  xsluo@nuist.edu.cn
• Federal Fiscal Year:
  2021
• Performing Organization:
  University of California, Berkeley
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Chemosphere
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7043016c64f7ed7db1fdb7403e392b93f3602cdb989b478ed2673d6e8bde9ee3aada9cff5397cf3df4f033fc88c3244bfda3714b220ce3e404e37f2c28e88179
• Download URL:
  https://stacks.cdc.gov/view/cdc/249487/cdc_249487_DS1.pdf
• File Type:
  [PDF - 5.56 MB ]