Short report: using targeted urine metabolomics to distinguish between manganese exposed and unexposed workers in a small occupational cohort.

Details

• Personal Author:
  Baker MG ; Carter KA ; Raftery D ; Simpson CD
• Description:
  Objectives: Despite the widespread use of manganese (Mn) in industrial settings and its association with adverse neurological outcomes, a validated and reliable biomarker for Mn exposure is still elusive. Here, we utilize targeted metabolomics to investigate metabolic differences between Mn-exposed and -unexposed workers, which could inform a putative biomarker for Mn and lead to increased understanding of Mn toxicity. Methods: End of shift spot urine samples collected from Mn exposed (n = 17) and unexposed (n = 15) workers underwent a targeted assay of 362 metabolites using LC-MS/MS; 224 were quantified and retained for analysis. Differences in metabolite abundances between exposed and unexposed workers were tested with a Benjamini-Hochberg adjusted Wilcoxon Rank-Sum test. We explored perturbed pathways related to exposure using a pathway analysis. Results: Seven metabolites were significantly differentially abundant between exposed and unexposed workers (FDR = 0.1), including n-isobutyrylglycine, cholic acid, anserine, beta-alanine, methionine, n-isovalerylglycine, and threonine. Three pathways were significantly perturbed in exposed workers and had an impact score >0.5: beta-alanine metabolism, histidine metabolism, and glycine, serine, and threonine metabolism. Conclusion: This is one of few studies utilizing targeted metabolomics to explore differences between Mn-exposed and -unexposed workers. Metabolite and pathway analysis showed amino acid metabolism was perturbed in these Mn-exposed workers. Amino acids have also been shown to be perturbed in other occupational cohorts exposed to Mn. Additional research is needed to characterize the biological importance of amino acids in the Mn exposure-disease continuum, and to determine how to appropriately utilize and interpret metabolomics data collected from occupational cohorts. [Description provided by NIOSH]
• Subjects:
  Biomarkers Energy Metabolism Manganese Occupational Exposure Occupational Health Safety Urine
• Keywords:
  Author Keywords: Occupational Health; Exposure Assessment; Targeted Metabolomics; Manganese Exposure; Biomonitoring Manganese; Biomarkers; Urine; Metabolites; Occupational Exposure; Exposure Assessment; Amino Acids; Biological Monitoring;
• ISSN:
  2296-2565
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• NIOSHTIC Number:
  nn:20064950
• Citation:
  Front Public Health 2021 May; 9:666787
• Contact Point Address:
  Marissa G. Baker, Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, United States
• Email:
  bakermg@uw.edu
• CAS Registry Number:
  Manganese (CAS RN 7439-96-5)
• Federal Fiscal Year:
  2021
• Performing Organization:
  University of Washington
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Frontiers in Public Health
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0c1dcfd7db2d2d2f16841dedc3da1fbc202afaa9d70f191f5ea262a0bb3cf85d6ab5d292a26d4a9cdd1b67fdf9d82e0804a73e322950678af745b75100da25e3
• Download URL:
  https://stacks.cdc.gov/view/cdc/249283/cdc_249283_DS1.pdf
• File Type:
  [PDF - 534.75 KB ]