Effect of inhalation exposure to cellulose nanocrystals on reproductive outcomes of male mice.

Details

• Personal Author:
  Guppi S ; Gutkin D ; Kisin ER ; McKinney W ; Shurin M ; Shvedova AA
• Description:
  Crystalline nanocelluloses (CNC) have good electrical, optical, and mechanical properties which make them desirable for industrial applications. We investigated adverse reproductive outcomes due to inhalation exposure to CNC aerosol, generated from a bulk supply of wood pulp derived cellulose nanocrystals. C57BL6/J male mice were exposed to precise concentration of airborne CNC (5 mg/m3, 5 h/day, 5 days/week for 1st and 2nd week and 4 days/week for 3rd week). Cauda epididymal sperm samples, testes and serum were collected to evaluate sperm alterations, oxidative stress, changes in the hormonal levels and inflammatory cytokine responses and perform testes histopathology at 24 h, 2-, 6- and 12-months post exposure. CNC inhalation significantly elevated abnormality in sperm heads and tail/mid-piece as well as reduced the number of motile sperm at all time points of recovery. Sperm DNA integrity assessed as DNA fragmentation index was significantly elevated only 24h post CNC inhalation and then reduced to the air-control level. Interstitial edema and occasional dystrophic seminiferous tubules with arrested spermatogenesis and degenerating spermatocytes were found in testes 6- and 12-month post inhalation while no changes were seen at the early time points. CNC inhalation produced significant imbalance in the levels of testosterone (throughout the recovery time of 2-12 month) and luteinizing hormone (only at 12-month post exposure). Assessment of testicular oxidative damage showed significantly higher amounts of protein carbonyls at all time points of recovery. A hierarchical cluster analysis of 23 cytokines/chemokines/growth factors of the testes separated inflammatory cytokines (G-CSF, IL-6, IL-12p70 and MIP-1a) and revealed patterns that differentiate early responses from later time points. Serum cytokines at the later time points clustered together in the close proximity to the 24h acute response. Overall, these results demonstrate that CNC inhalation exposure induces sustained male reproductive toxicity observed up to 12 months of recovery. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Electricity Endocrine System Environmental Exposure Laboratories Occupational Health Respiratory System Risk Assessment Risk Factors Safety Spermatogenesis Urogenital Diseases Urogenital System

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• Keywords:
  Electrical properties; Mechanical properties; Reproductive effects; Reproductive system disorders; Inhalation; Exposure levels; Risk factors; Aerosols; Sampling; Oxidative stress; Hormones; Cytokines; Histopathology; DNA; Deoxyribonucleic acids; Spermatogenesis; Animals; Laboratory animals
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  186
• NIOSHTIC Number:
  nn:20064944
• Citation:
  Toxicologist 2022 Mar; 186(S1):218
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 61st Annual Meeting & ToxExpo, March 27-31, 2022, San Diego, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e2583bfe0393353c87b089e11d7b1097f4a69b24ec6cbe066f5f71d4d9475bf8a4c7c70b6761c14a5fde500d07e0ce2cc85b7b170b3a8b9c596291ca57085161
• Download URL:
  https://stacks.cdc.gov/view/cdc/249280/cdc_249280_DS1.pdf
• File Type:
  [PDF - 222.40 KB ]