Impacts of a nasal rinse on inflammation and microbiome diversity in dairy workers.

Details

• Personal Author:
  Erlandson G ; Magzamen S ; Reynolds S ; Schaeffer J
• Description:
  Livestock workers experience an increased burden of bioaerosol-induced respiratory disease including high prevalence of asthma and rhinosinusitis. Dairy farms generate high concentrations of bioaerosols that span the inhalable size range (0-100 µm), which can carry diverse microbial communities (including pathogens and inflammagens). Because larger particles impact in the nasopharyngeal region, we evaluated the effectiveness of a hypertonic saline nasal rinse in reducing inflammatory responses in the upper airways in dairy workers. Additionally, we investigated potential treatment impacts on the nasal microbiome. Pre- and post-shift nasal lavage samples were collected from each participant over five consecutive days. Treatment group participants received a hypertonic saline rinse while control group participants received normotonic saline rinse. Pro- and anti-inflammatory cytokines (i.e., IL-8, IL-10, and TNF-a) were measured from lavage samples using a multiplex assay. Nasal microbiomes were characterized using 16S rRNA sequencing on lavage samples. We present comparisons of inflammatory cytokines between treatment and control groups in the context of a statistical model. Distinct bacterial communities were observed in pre- and post-shift lavage samples indicating bacterial uptake from the dairy environment. Using Faith's phylogenetic diversity metric, we observed higher alpha diversity in the treatment group compared to the control. However, statistical analysis indicated the difference was not significant. Weighted UniFrac Beta diversity tests indicate that there was not a significant difference in beta diversity between groups. These results demonstrate that use of a nasal rinse as a low-cost, non-invasive intervention to reduce inflammation among bioaerosol-exposed dairy workers does not negatively impact microbial diversity. [Description provided by NIOSH]
• Subjects:
  Immune System Livestock Nose Occupational Health Safety Sense Organs
• Keywords:
  Livestock industry; Animal husbandry workers; Bioaerosols; Nasal cavity; Intervention effectiveness; Immune reaction
• Publisher:
  International Society of Exposure Science
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  205-206
• NIOSHTIC Number:
  nn:20070938
• Citation:
  International Society of Exposure Science 2022 Annual Meeting (ISES 2022): From Exposure to Human Health: New Developments and Challenges in a Changing Environment, September 25-29, 2022, Lisbon, Portugal. Ottawa, Canada: International Society of Exposure Science, 2022 Sep; :205-206
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  False
• Start Date:
  20030915
• Source Full Name:
  International Society of Exposure Science 2022 Annual Meeting (ISES 2022): From Exposure to Human Health: New Developments and Challenges in a Changing Environment, September 25-29, 2022, Lisbon, Portugal
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/248333/cdc_248333_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]