Do certain nasal microbiome characteristics correlate to viral or MRSA susceptibility in dairy workers?

Details

• Personal Author:
  Erlandson G ; Leibler J ; Reynolds S ; Schaeffer J ; Seidel J
• Description:
  Livestock workers are exposed to bioaerosols comprised of diverse bacterial and viral constituents. Consequently, opportune pathogens present in these bioaerosols may infect workers. Pathogens identified at dairy farms include influenzas (e.g., the novel influenza D virus), coronaviruses, livestock- and community-associated methicillin-resistant staphylococcus aureus (MRSA). The nasal microbiome in livestock workers may play a role in the carriage of pathogens. Here, we analyzed 237 nasal lavage samples from 31 dairy workers to determine the carriage of Influenzas A, C, and D, methicillin- susceptible staphylococcus aureus (MSSA), and MRSA. Using Illumina technology, we characterized participants' nasal microbiome, specifically bacterial communities. Amplicon sequence variants were constructed using a GreenGenes pre-trained classifier. Diversity metrics based on pathogens of interest were performed using QIIME2's core-metrics pipeline. Analysis of the nasal lavages revealed 2.5% were positive for Influenza A, 1.3% positive for Influenza C, 17.3% positive for Influenza D, 1.3% positive for MRSA, and 32.5% positive for MSSA. Alpha diversity was explored using Shannon Diversity Index, and pairwise Kruskal-Wallis comparisons of lavages found no significant differences in alpha diversity based on individual pathogens. PERMANOVA based on weighted UniFrac distances was performed to determine differences in beta diversity. A significant difference (p=0.01) in beta diversity was observed between lavages testing positive and negative for MSSA. We demonstrate that: pathogens occupy the nares of dairy workers during their shift, and a greater taxonomic diversity may confer protection against MSSA infection/carriage. Planned analysis of lavages should elicit a deeper understanding of the nasal microbiome characteristics that protect workers. [Description provided by NIOSH]
• Subjects:
  Livestock Nose Occupational Health Safety Sense Organs Viral Diseases
• Keywords:
  Livestock industry; Animal husbandry workers; Bioaerosols; Nasal cavity; Pathogens; MRSA; Methicillin resistant Staphylococcus aureus; Viral infections
• Publisher:
  International Society of Exposure Science
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Colorado ; Massachusetts ; OSHA Region 1 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  249-250
• NIOSHTIC Number:
  nn:20070939
• Citation:
  International Society of Exposure Science 2022 Annual Meeting (ISES 2022): From Exposure to Human Health: New Developments and Challenges in a Changing Environment, September 25-29, 2022, Lisbon, Portugal. Ottawa, Canada: International Society of Exposure Science, 2022 Sep; :249-250
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  False
• Start Date:
  20030915
• Source Full Name:
  International Society of Exposure Science 2022 Annual Meeting (ISES 2022): From Exposure to Human Health: New Developments and Challenges in a Changing Environment, September 25-29, 2022, Lisbon, Portugal
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/248334/cdc_248334_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]