Arachidonic acid supplementation enhances hydrogen peroxide induced oxidative injury of neonatal rat cardiac myocytes.

Details

• Personal Author:
  Hoffman P ; Plews P ; Toraason M ; Wey H ; Woolery M
• Description:
  Rat cardiac myocyte sensitivity to hydrogen-peroxide (7722841) (HP) induced oxidative injury was investigated to determine the effect of variations in phospholipid arachidonic-acid content. Arachidonic- acid supplements of 0, 50, or 100 micromoles (micromol) were administered to cultured cardiac ventricular monocytes from 2 to 4 day old Sprague-Dawley-rats for 36 hours, then the cells were exposed to HP concentrations of 50 or 100microM for 1 hour. Following exposure, cells were subjected to phospholipid fatty acid analysis. Cytotoxicity was determined via lactate-dehydrogenase assay. Thiobarbituric-acid reactive substance assay was performed on cells to assess lipid peroxidation. Arachidonic-acid supplementation produced a 55% increase in the arachidonic-acid content of myocyte phospholipids. Concentration dependent increases in cardiac myocyte lipid peroxidation and cytotoxicity were observed after HP exposure, with enhanced effects seen in cells supplemented with 100microM arachidonic-acid for 36 hours. Marked cytotoxicity and lipid peroxidation was observed in cultured myocytes to which unesterified arachidonic-acid was added. This effect was prevented by addition of 10microM vitamin-E or 1 millimolar desferrioxamine. The authors conclude that cardiac myocyte arachidonic-acid supplementation enhances myocyte susceptibility to HP induced oxidative injury. [Description provided by NIOSH]
• Subjects:
  Antioxidants Cells, Cultured Cytotoxins Hydrogen Peroxide Laboratories Occupational Health Peroxides Safety
• Keywords:
  Author Keywords: Cardiomyocyte; Arachidonic Acid; Hydrogen Peroxide; Oxidative Injury; Peroxidation; Fatty Acid Composition; Antioxidants; Aspirin NIOSH-Author; Cell-function; Mammalian-cells; In-vitro-studies; Laboratory-animals; Oxidative-processes; Cytotoxic-effects; Fatty-acids; Antioxidants; Peroxides; Cell-cultures;
• ISSN:
  0008-6363
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  29
• Issue:
  5
• NIOSHTIC Number:
  nn:00238996
• Citation:
  Cardiovasc Res 1995 May; 29(5):624-628
• CAS Registry Number:
  Hydrogen peroxide (CAS RN 7722-84-1)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Cardiovascular Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247909/cdc_247909_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]