Nitric oxide synthase inhibitor and lipopolysaccharide effects on reactivity of guinea pig airways.

Details

• Personal Author:
  Fedan, Jeffery S. ; Frazer DG ; Robinson VA ; Warner TE ; Yuan LX
• Description:
  Nitric-oxide-synthase inhibitors and lipopolysaccharides (LPS) were tested on guinea-pig tracheas. Each trachea sample was mounted to a perfusion holder and connected to a differential pressure transducer via side hole catheters. Concentration response curves were generated by adding increasing, cumulative amounts of the various inhibitors to the tracheal baths. The inhibitors N-omega-nitro-L- arginine-methyl-ester (L-NAME), NG-methyl-L-arginine (L-NMMA), and aminoguanidine were tested on trachea already responding to methacholine or histamine. These inhibitors did not affect the pressure differences across the trachea, nor did they increase contractile responses to methacholine or histamine. These facts suggested that guinea-pig airways were not influenced by nitric- oxide (10102439) (NO). However, NO production was up regulated by the addition of LPS, observed in the decreased tracheal reactivity to both methacholine and histamine in the presence of LPS. Also, L- NAME increased reactivity to methacholine with LPS present in the tracheal baths. LPS only affected responses to histamine when the epithelium was present, suggesting that histamine response was related to the epithelium and methacholine response, since it occurred with or without the epithelium present, was related to smooth muscle. The authors conclude that lipopolysaccharides induce nitric-oxide-synthase, since addition of L-NAME in the presence of LPS potentiates contractions caused by methacholine. Without LPS present, NO does not appear to have a major role in regulating guinea-pig airway reactivity. [Description provided by NIOSH]
• Subjects:
  Laboratories Nitrogen Oxides Occupational Health Respiratory System Safety
• Keywords:
  NIOSH-Author; Nitrogen-oxides; Airway-resistance; Laboratory-animals; Muscle-contraction; Enzyme-inhibitors; In-vivo-studies
• ISSN:
  0022-3565
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  272
• Issue:
  3
• NIOSHTIC Number:
  nn:00232189
• Citation:
  J Pharmacol Exp Ther 1995 Mar; 272(3):1141-1150
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Pharmacology and Experimental Therapeutics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247843/cdc_247843_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]