Alterations of histone phosphorylation in rat spleen cells after treatment with the aromatic amine, 4,4'-methylene-bis(2-chloroaniline).

Details

• Personal Author:
  Booth-Jones AD ; Cheever KL ; DeBord, D. Gayle ; Savage RE Jr. ; Swearengin TF
• Description:
  A study was conducted on the effects of 4,4'-methylene-bis-(2- chloroaniline) (95534) (MOCA) on histone phosphorylation in rodent spleen cells. Spleen cells isolated from Sprague-Dawley-rats were incubated with up to 50 micromolar (microM) MOCA and the five major histone groups were evaluated using polyacrylamide gel electrophoresis. A significant increase in histone phosphorylation was seen following exposure to 10microM MOCA. MOCA incubation resulted in an initial transient decrease in histone phosphorylation followed by stimulation. The histone H2B group was least affected of all the histones to MOCA induced alterations. The degree of phosphorylation was unaffected by increasing MOCA concentrations with higher concentrations resulting in normal histone phosphorylation values for all histone groups with the exception of H3, which remained elevated following exposure to concentrations greater than 10microM. [Description provided by NIOSH]
• Subjects:
  Amines Aniline Compounds Carcinogens Chlorine Compounds Occupational Health Safety
• Keywords:
  NIOSH-Author; Carcinogens; Anilines; Chlorine-compounds; Amines; Protein-biochemistry; Oxidative-phosphorylation; Arylamines
• ISSN:
  0887-2082
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  19-23
• Volume:
  10
• Issue:
  1
• NIOSHTIC Number:
  nn:00226575
• Citation:
  J Biochem Toxicol 1995 Feb; 10(1):19-23
• CAS Registry Number:
  o-Toluidine (CAS RN 95-53-4)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Biochemical Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247814/cdc_247814_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]