Macromolecular adduct formation by 4,4'-methylene-bis(2-chloroanaline) in adult male rat.

Details

• Personal Author:
  Begley KB ; Cheever KL ; Daniel FB ; Richards, Donald E. ; Savage RE Jr. ; Weigel WW
• Description:
  Formation of macromolecular 4,4'-methylene-bis(2-chloroaniline) (101144) (MOCA) adducts was studied in rats. Male Sprague-Dawley- rats were given a single oral dose of 0 or 281 micromoles per kilogram carbon-14 (C-14) labeled MOCA, and killed 1, 3, 7, 10, 14, or 29 days after dosing. The livers were taken and the blood collected. C-14 activity in the blood and liver was measured. C-14 binding to globin, hemin, and erythrocyte cell debris in the blood and hepatic DNA was determined. The amounts of radiolabel found in the whole blood, liver, hemin, erythrocyte cell debris, and globin after 24 hours were 3417, 5181, 646, 2912, and 3595 femtomoles per milligram, respectively. The ratio of C-14 in liver DNA to whole liver C-14 activity increased from 4.3 after 1 day to 70.8 after 29 days. The ratio of liver DNA/globin C-14 activity, 5.4, did not change significantly over the 29 day period. The biological halflives for MOCA derived C-14 activity in the globin, hepatic DNA, hemin, liver, whole blood, and erythrocyte cell debris were 14.3, 11.1, 8.6, 4.4, 16.7, and 27.4 days, respectively. The authors conclude that since the biological halflives for rat globin and liver DNA/MOCA adducts are similar and the DNA/globin C-14 activity ratio does not change very much over time, quantitating globin/MOCA adducts may be a useful technique for monitoring exposure to MOCA. [Description provided by NIOSH]
• Subjects:
  Aniline Compounds Chlorine Compounds Laboratories Occupational Health Safety
• Keywords:
  Anilines Chemical-binding In-vivo-studies Laboratory-animals NIOSH-Author Nucleic-acids Organo-chlorine-compounds Pharmacodynamics Physiological-chemistry
• ISSN:
  0355-3140
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  57-59
• Volume:
  14
• NIOSHTIC Number:
  nn:00181597
• Citation:
  Scand J Work Environ Health 1988 May/Jun; 14(Suppl 1):57-59
• Contact Point Address:
  National Institute for Occupational Safety and Health, Cincinnati. Ohio, USA
• CAS Registry Number:
  3,3′-Dichloro-4,4′-diaminodiphenylmethane (CAS RN 101-14-4)
• Federal Fiscal Year:
  1988
• Peer Reviewed:
  True
• Source Full Name:
  Scandinavian Journal of Work, Environment & Health
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0ebb23d85faadfa5291be02c80f2acd7dcc652b5758f9e3b50c4dfd84a470664fe2a7aab9ea65f272e6212d0de36c450d25decbf40cdb5add6186e76d9fd848e
• Download URL:
  https://stacks.cdc.gov/view/cdc/185605/cdc_185605_DS1.pdf
• File Type:
  [PDF - 1.55 MB ]