Micronuclei assay in cytokinesis-blocked binucleated and conventional mononucleated methods in human peripheral lymphocytes.

Details

• Personal Author:
  Channarayappa JNa ; Ong T
• Description:
  The effect of cytochalasin-B (14930962) (CYB) on the induction of chromosomal aberrations, and the frequencies of micronuclei induced by treatment of cells with mitomycin-C (50077) (MMC) and cyclophosphamide (50180) (CPA), were examined. Lymphocytes from a normal individual were isolated, cultured for 48 hours and exposed to 1, 2, 3, 4, 5.5, 7.5, or 10 micrograms/milliliter (microg/ml) for 24 hours. Certain cultures were also exposed to MMC after 40 hours of incubation, or incubated with CPA for 3 hours, washed and incubated with 3microg/ml CYB for an additional 24 hours. Cells were harvested, fixed, stained and examined for micronuclei. A CYB dose dependent increase in micronuclei was seen, with peak numbers of binucleated and multinucleated cells occurring with doses of 3 to 4microg/ml CYB. Both MMC and CPA treatment resulted in dose dependent increases in micronuclei frequencies when tested on the CYB cytokinesis blocked binucleated cells and on conventional mononuclear cells. [Description provided by NIOSH]
• Subjects:
  Cell Division Cells, Cultured Chromosomes Hazardous Substances Laboratories Occupational Health Safety
• Keywords:
  Author Keywords: Micronucleus Assay; Human Lymphocytes; Cytochalasin-B; Binucleated Cell; Mononucleated Cell NIOSH-Author; Cell-cultures; Cell-division; Chromosome-damage; Toxic-effects; Dose-response; Analytical-methods; Laboratory-techniques;
• ISSN:
  0270-3211
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  273-279
• Volume:
  10
• Issue:
  3
• NIOSHTIC Number:
  nn:00204966
• Citation:
  Teratog, Carcinog, Mutagen 1990 Jan; 10(3):273-279
• Contact Point Address:
  T. Ong, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 944 Chestnut Ridge Road, Morgantown, West Virginia 26505-2888
• CAS Registry Number:
  Cyclophosphamide (CAS RN 50-18-0) ; Cytochalasin B (CAS RN 14930-96-2) ; Mitomycin C (CAS RN 50-07-7)
• Federal Fiscal Year:
  1990
• Peer Reviewed:
  True
• Source Full Name:
  Teratogenesis, Carcinogenesis, and Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247745/cdc_247745_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]