Vanadate-Induced Cell Growth Regulation and the Role of Reactive Oxygen Species

Details

• Personal Author:
  Butterworth L ; Huang C ; Lanciotti R ; Leonard, Stephen S. ; Li J ; Shi X ; Zhang Z
• Description:
  While vanadium compounds are known as potent toxicants as well as carcinogens, the mechanisms of their toxic and carcinogenic actions remain to be investigated. It is believed that an improper cell growth regulation leads to cancer development. The present study examines the effects of vanadate on cell cycle control and involvement of reactive oxygen species (ROS) in these vanadate-mediated responses in a human lung epithelial cell line, A549. Under vanadate stimulation, A549 cells generated hydroxyl radical (OH), as determined by electron spin resonance (ESR), and hydrogen peroxide (H2O2) and superoxide anion (O2-), as detected by flow cytometry using specific dyes. The mechanism of ROS generation involved the reduction of molecular oxygen to O2- by both a flavoenzyme-containing NADPH complex and the mitochondria electron transport chain. The O2- in turn generated H2O2, which reacted with vanadium(IV) to generate OH radical through a Fenton-type reaction (V(IV) + H2O2 --> V(V) +OH + OH-). The ROS generated by vanadate induced G2/M phase arrest in a time- and dose-dependent manner as determined by measuring DNA content. Vanadate also increased p21 and Chk1 levels and reduced Cdc25C expression, leading to phosphorylation of Cdc2 and a slight increase in cyclin B1 expression as analyzed by Western blot. Catalase, a specific antioxidant for H2O2, decreased vanadate-induced expression of p21 and Chk1, reduced phosphorylation of Cdc2Tyr15, and decreased cyclin B1 levels. Superoxide dismutase, a scavenger of O2-, or sodium formate, an inhibitor of OH, had no significant effects. The results obtained from the present study demonstrate that among ROS, H2O2 is the species responsible for vanadate-induced G2/M phase arrest. Several regulatory pathways are involved: (1) activation of p21, (2) an increase of Chk1 expression and inhibition of Cdc25C, which results in phosphorylation of Cdc2 and possible inactivation of cyclin B1/Cdc2 complex. [Description provided by NIOSH]
• Subjects:
  Antioxidants Carcinogens Cell Division Cells, Cultured Hazardous Substances Neoplasms Occupational Health Safety Vanadium Compounds
• Keywords:
  Author Keywords: Vanadate Cancer Carcinogenesis Carcinogenicity Cdc2 Cdc25C Cell-biology Cell-cultures Cell-division Cell-growth Cell Cycle Cellular-function Chk1 Cyclin B1 P21 Reactive Oxygen Species; Toxic-effects Toxins Vanadium-compounds

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• ISSN:
  0003-9861
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  311-320
• Volume:
  392
• Issue:
  2
• NIOSHTIC Number:
  nn:20021941
• Citation:
  Arch Biochem Biophys 2001 Aug; 392(2):311-320
• Email:
  xas0@cdc.gov
• CAS Registry Number:
  Sodium vanadium oxide (Na3VO4) (CAS RN 13721-39-6) ; Vanadium (CAS RN 7440-62-2)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Archives of Biochemistry and Biophysics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3bd8402b426450d6f9522ce58c09c57e299dbc000ffd171aefbe401dfd0670008af07d9f1543c3121cd9ce97e3c7856b414fc8c81ee11143d618d4b0fad14e41
• Download URL:
  https://stacks.cdc.gov/view/cdc/199015/cdc_199015_DS1.pdf
• File Type:
  [PDF - 217.00 KB ]