Histopathological assessment of triphenyl phosphite neurotoxicity in the hen.

Details

• Personal Author:
  Abou-Donia MB ; Brown HR ; Carrington CD
• Description:
  The clinical and histopathological effects of triphenyl-phosphite (115866) (TPP) were examined in a dose and time dependent manner. A comparison was also made of the neurotoxicity of TPP to the effects of diisopropyl-phosphorofluoridate (55914) (DFP) and tri-o-cresyl- phosphate (78308) (TOCP). The ability of phenylmethylsulfonyl- fluoride (PMSF) to prevent the neuropathies induced by subcutaneous doses of either TPP or TOCP was studied and the ability of DFP to cause additional damage after exposure to TPP was investigated. White-leghorn-hens were treated with TPP doses of 250, 500, or 1000mg/kg and evaluation for pathological changes was conducted 21 days later. In the comparison studies, TOCP was administered at a dose of 1187mg/kg and DFP at 1.7mg/kg. All three chemicals were administered subcutaneously in the neck. Progressive ataxia and paralysis developed 5 to 10 days after dosing with TPP at 1000mg/kg. Axonal damage in the lateral columns of the spinal cord and peripheral nerve accompanied the clinical signs. Similar signs followed neurotoxic doses of TOCP or DFP. TPP caused damage to axons in the brain and gray matter of the spinal cord with chromatolysis and neuronal necrosis observed in the spinal cord; these effects were not observed following treatment with TOCP or DFP. All histopathologic changes observed with TPP were dose dependent. The minimum neurotoxic dose of TPP was 500mg/kg. The incidence of damage to the peripheral nerve of TPP treated animals was reduced by prior administration of 30mg/kg PMSF. However, PMSF did not prevent toxicity to the cell bodies in the spinal cord or the clinical effects. The authors conclude that TPP caused neuronal damage in addition to the axonal damage associated with organophosphorous compound induced delayed neuropathy, and suggest that two distinct mechanisms underlie the neurotoxicity of TPP. [Description provided by NIOSH]
• Subjects:
  Agriculture Insecticides Nervous System Nervous System Diseases Occupational Health Organophosphates Safety
• Keywords:
  NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Agricultural-chemicals; Organo-phosphorus-compounds; Organo-phosphorus-pesticides; Histopathology; Cell-damage; Nerve-tissue; Nervous-system-disorders
• ISSN:
  0161-813X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-234
• Volume:
  9
• Issue:
  2
• NIOSHTIC Number:
  nn:00185864
• Citation:
  Neurotoxicology 1988 Mar; 9(2):223-234
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  Diisopropyl fluorophosphate (CAS RN 55-91-4) ; o-Cresyl phosphate (CAS RN 78-30-8) ; Triphenyl phosphate (CAS RN 115-86-6)
• Federal Fiscal Year:
  1988
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Neurotoxicology
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247670/cdc_247670_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]