Role of gap junctions in adverse reproductive outcome.

Details

• Personal Author:
  Caruso RL
• Description:
  Several toxicants were evaluated for their effects on junctional communication between cells. Various techniques were used to measure junctional communication, including a new, rapid and sensitive dye coupling assay. The V79 cell line was used in most of the experiments, derived from Chinese-hamster embryo lung, along with the HT cell line, derived from a human teratocarcinoma and the SK-UT-1 cell line, derived from a human leiomyosarcoma of the uterus. Responses to reproductive and developmental toxicants including dieldrin (60571), ethylene-glycol-monomethyl-ether, ethylene-glycol-monoethyl-ether, ethanol (64175), lead (7439921), mercury (7439976), nickel (7440020), zinc (7440666), and arsenic (7440382) were monitored. Junctional communication was inhibited in at least one of the cell lines by every substance tested except nickel and zinc. Work has begun to determine the effects of calcium (7440702) and cAMP on junctional communication in the SK-UT-1 cells. The findings suggest that cAMP may modulate gap junctions by multiple mechanisms, including the synthesis of new protein. Studies also used the hydra, a simple invertebrate, to evaluate junctional communication in a whole animal, morphogenetic system. [Description provided by NIOSH]
• Content Notes:
  in NTRL, no pdf
• Subjects:
  Agriculture Chlorine Compounds Insecticides Metals Occupational Health Poisoning Safety Solvents Urogenital Diseases
• Keywords:
  NIOSH-Grant; Reproductive-system-disorders; Organo-chlorine-compounds; Pesticides; Agricultural-chemicals; Solvents; Metallic-poisoning
• Series:
  Grant Final Reports
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Final Grant Report
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OEP - Office of Extramural Programs
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-26
• NIOSHTIC Number:
  nn:00185292
• NTIS Accession Number:
  PB89164743
• Citation:
  University of Michigan, Ann Arbor, Michigan 1988 Nov; :1-26
• Contact Point Address:
  Environmental & Indust Health University of Michigan 109 Observatory Street Ann Arbor, MI 48109-2029
• CAS Registry Number:
  Arsenic (CAS RN 7440-38-2) ; Calcium (CAS RN 7440-70-2) ; Dieldrin (CAS RN 60-57-1) ; Ethanol (CAS RN 64-17-5) ; Lead (CAS RN 7439-92-1) ; Mercury (CAS RN 7439-97-6) ; Nickel (CAS RN 7440-02-0) ; Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  1989
• NORA Priority Area:
  Reproductive System Disorders
• Performing Organization:
  University of Michigan at Ann Arbor, Ann Arbor, Michigan
• Peer Reviewed:
  False
• Start Date:
  19860201
• Source Full Name:
  University of Michigan, Ann Arbor, Michigan
• End Date:
  19870831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/246180/cdc_246180_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]