Immunologic methods for the detection of human exposure to polycyclic aromatic hydrocarbons.

Details

• Personal Author:
  Gomes M ; Perera FJ ; Santella RM ; Young TL ; Zhang YJ
• Description:
  Immunologic methods for detecting exposure to polycyclic aromatic hydrocarbons (PAHs) were discussed. PAH biological monitoring research has focused on determining the concentrations of PAH/DNA and PAH/protein adducts. PAH/DNA adduction is of major interest since cellular DNA is the target of carcinogens. PAH/protein adducts are regarded as surrogates for PAH/DNA adduction. The focus of the research has been on developing methods for measuring DNA and protein adduction by benzo(a)pyrene (50328) (BaP), a prototype PAH. To detect BaP/DNA adducts, monoclonal and polyclonal antisera have been developed against benzo(a)pyrene-diol-epoxide (BPDE)/DNA. When used with an enzyme linked immunosorbent assay (ELISA), sensitivities on the order of one femtomole (fmol) have been obtained. The results of studies of PAH/DNA adducts in various occupationally and environmentally exposed populations were discussed. Studies in coke oven workers have found WBC BaP/DNA adduct concentrations ranging from 0.80 to 5.0 adducts per 10(7) nucleotides. The adduct levels were correlated with the levels of PAH exposure. Detecting BPDE/protein adducts was discussed. Hemoglobin and albumin have been used in studies of PAH/protein adduction. An assay utilizing a monoclonal antibody developed from animals immunized with BPDE/guanosine conjugated with bovine serum albumin has been developed. Studies of serum albumin adduction in coke oven workers have found PAH/albumin levels of 5.22fmol/microgram, versus 4.07fmol/microgram in unexposed persons. Urinary PAH excretion as a marker of exposure was discussed. Urinary excretion of PAHs by patients treated with coal-tar for psoriasis has been measured with an ELISA. [Description provided by NIOSH]
• Subjects:
  Biochemistry Dermatitis Environmental Exposure Hydrocarbons Immune System Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Risk Assessment Safety
• Keywords:
  NIOSH-Grant; Polycyclic-aromatic-hydrocarbons; Biochemical-indicators; DNA-adducts; Immunochemistry; Hemoproteins; Risk-analysis; Dermatitis; Biological-monitoring; Coke-oven-workers; Occupational-exposure; Environmental-exposure
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Proceedings
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  81-94
• NIOSHTIC Number:
  nn:00232202
• Citation:
  Health Risk Assessment and Surveillance in the Industrial Setting, Symposium Proceedings, May 27-29, 1991, Tapei, Taiwan. 1992 Apr; :81-94
• Contact Point Address:
  Environmental Sciences Columbia University 650 West 168Th Street New York, NY 10032
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  1992
• Performing Organization:
  Columbia University New York, New York, New York
• Peer Reviewed:
  False
• Start Date:
  19890101
• Source Full Name:
  Health Risk Assessment and Surveillance in the Industrial Setting, Symposium Proceedings, May 27-29, 1991, Tapei, Taiwan
• End Date:
  19921231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/243454/cdc_243454_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]