U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Improved methods of producing precipitating Aspergillus antigens.



Details

  • Personal Author:
  • Description:
    Improved methods for the production of Aspergillus-fumigatus antigens which are capable of giving sensitive and reproducible results by counterimmunoelectrophoresis (CIEP) were described. The influence of the type of casein hydrolysate used, the influence of the method of incubating the CIEP slide, the effect of freeze pressing and concentrating antigen preparations, the effect age of culture, and the effect of carbon and nitrogen source concentrations on antigen production were examined. Of the three different commercial sources used, polypeptone, neopeptone, and multi-peptone, only the antigen produced with polypeptone formed at least four lines without concentration when tested by CIEP with the reference antiserum. The other two antigens formed no discernible lines on CIEP. With regard to incubation time, several more fine lines were apparent when incubation was extended to 18 hours at 4 degrees-C. Concentrating the clarified extract five fold produced a few more lines but, more importantly, the procedure resulted in stronger lines of precipitation, particularly among the minor antigen antibody reaction lines. Older cultures resulted in preparations yielding fewer lines than younger cultures. The authors conclude that reliable Aspergillus antigens can easily be produced in the clinical laboratory. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0003-4738
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    202-205
  • Volume:
    38
  • Issue:
    3
  • NIOSHTIC Number:
    nn:00189166
  • Citation:
    Ann Allergy 1977 Mar; 38(3):202-205
  • Contact Point Address:
    Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
  • Federal Fiscal Year:
    1977
  • NORA Priority Area:
    Pulmonary System Disorders
  • Performing Organization:
    West Virginia University, Morgantown, West Virginia
  • Peer Reviewed:
    True
  • Start Date:
    19710601
  • Source Full Name:
    Annals of Allergy
  • End Date:
    19830831
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
  • Download URL:
  • File Type:
    Filetype[PDF - 168.02 KB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP