In Vivo Analysis of UV-B Induced Photooxidations.

Details

• Personal Author:
  Krinsky NI
• Description:
  Attempts were made to clarify the initial photochemical mechanism by which ultraviolet-B (UV-B) irradiation acts in-vivo. The knowledge already gained of the photochemical and oxidative reactions of cholesterol were used in accomplishing this objective. The Skh-hr-1- hairless-mouse was used as the test system to evaluate the primary process of UV-B photooxidation in-vivo. The animals were injected with tritium labeled mevalonic-acid to produce radioactive cholesterol in-vivo. Injections were made 1 hour prior to irradiation. The major radioactive lipid expected to be formed was cholesterol. Preliminary experiments indicated that ultraviolet irradiation resulted in the formation of a compound or compounds more polar than cholesterol. Products which were expected to be formed other than hydroperoxides were to be investigated in future studies. This included cholesterol-5,6-epoxide and its product, 3beta,5alpha,6beta-trihydroxycholesterol. Studies will also be extended to the Skh-crh-mouse strain which has a low incidence of UV- B induced skin tumors. [Description provided by NIOSH]
• Subjects:
  Carcinogens Energy Metabolism Laboratories Occupational Health Radiation Safety Skin Ultraviolet Rays
• Keywords:
  Laboratory-animals; Ultraviolet-radiation; Radiation-exposure; Skin-exposure; Carcinogenesis; Metabolic-study; Photochemical-reactions; Nonionizing-radiation
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:00187801
• Citation:
  NIOSH 1982 Apr:768-771
• Federal Fiscal Year:
  1982
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the Second NCI/EPA/NIOSH Collaborative Workshop: Progress on Joint Environmental and Occupational Cancer Studies, September 9-11, 1981, Rockville, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242795/cdc_242795_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]