Uroporphyrin I stimulation of collagen biosynthesis in human skin fibroblasts. A unique dark effect of porphyrin

Details

• Personal Author:
  Bickers DR ; Schiltz JR ; Varigos G
• Description:
  A study was undertaken to assess the effects of uroporphyrin-I and of light on collagen synthesis in fibroblasts obtained from explant cultures of normal adult human skin. Fibroblast monolayers were preincubated for 24 hours in the dark with or without uroporphyrin-I and then incubated for 24 hours with tritium labeled proline. Accumulation of nondialyzable isotopically labeled hydroxyproline increased 2.7 fold when uroporphyrin-I was included in the incubation medium. This represented a specific increase in collagen synthesis. This increase was time dependent and occurred either in the presence or absence of ultraviolet light. A study was performed to determine whether the increased collagen accumulation in the presence of uroporphyrin-I might be the result of decreased collagen degradation. The study of the effects of uroporphyrin-I and light on the growth of human fibroblasts indicated the growth curves were similar for all cultures including the controls, light without porphyrin, porphyrin without light and porphyrin irradiated daily with 400 nanometer light. There were no obvious differences in cellular morphology in the cultures when examined by phase contrast microscopy. The authors suggest that the sclerodermatous lesions seen in the skin of patients with porphyria cutanea tarda and erythropoietic porphyria may be the result of the novel dark effect of uroporphyrin-I as demonstrated in this study. [Description provided by NIOSH]
• Subjects:
  Dermatitis Energy Metabolism Liver Occupational Health Radiation Safety Skin Ultraviolet Rays
• Keywords:
  In-vitro-studies Liver-disorders Metabolic-study NIOSH-Grant NIOSH-Publication Skin-exposure Ultraviolet-radiation
• ISSN:
  0021-9738
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  129-135
• Volume:
  69
• Issue:
  1
• NIOSHTIC Number:
  nn:00187020
• Citation:
  J Clin Invest 1982 Jan; 69(1):129-135
• Contact Point Address:
  Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
• Federal Fiscal Year:
  1982
• NORA Priority Area:
  Disease and Injury: Allergic and Irritant Dermatitis
• Performing Organization:
  Case Western Reserve University, Cleveland, Ohio
• Peer Reviewed:
  True
• Start Date:
  19800929
• Source Full Name:
  Journal of Clinical Investigation
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:481ea3ba9673177d20477d2f34a832816b63be568c20420979bffcaac7607f45b12cf9b28f6a7fba055a39646a5b98ba285f1447a97e3ea1d9649cf2ee12b741
• Download URL:
  https://stacks.cdc.gov/view/cdc/205832/cdc_205832_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]