Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay
Published Date:Dec 2011
Source:Emerg Infect Dis. 17(12):2277-2286.
Amino Acid Sequence
Communicable Diseases, Emerging
Democratic Republic Of The Congo
High-Throughput Screening Assays
Molecular Sequence Data
Multiplexed Antibody Detection Assay
Simian Acquired Immunodeficiency Syndrome
Simian Immunodeficiency Virus
Funding:R01 AI 50529/AI/NIAID NIH HHS/United States
Description:Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.
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