Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Ahuka-Mundeke, Steve ; Ayouba, Ahidjo ; Mbala-Kingebeni, Placide ; Liegeois, Florian ; Esteban, Amandine ; Lunguya-Metila, Octavie ; Demba, Didace ; Bilulu, Guy ; Mbenzo-Abokome, Valentin ; Inogwabini, Bila-Isia ; Muyembe-Tamfum, Jean-Jacques ; Delaporte, Eric ; Peeters, Martine
• Description:
  Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.
• Subjects:
  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral
  • Base Sequence
  • Colobus
  • Communicable Diseases, Emerging
  • Cross Reactions
  • DNA, Z-Form
  • Genetic Variation
  • Hiv Antibodies
  More +
• Source:
  Emerg Infect Dis. 17(12):2277-2286.
• Document Type:
  Journal Article
• Funding:
  R01 AI 50529/AI/NIAID NIH HHS/United States
• Place as Subject:
  Democratic Republic of the Congo
• Volume:
  17
• Issue:
  12
• Download URL:
  https://stacks.cdc.gov/view/cdc/23787/cdc_23787_DS1.pdf
• File Type:
  [PDF - 417.95 KB]
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:d55ae5e3d30bf28ab902b3882f6f35ee7f70d246d6eb03ee427b4a5b79fa11ef