Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Ahuka-Mundeke, Steve; Ayouba, Ahidjo; Mbala-Kingebeni, Placide; Liegeois, Florian; Esteban, Amandine; Lunguya-Metila, Octavie; Demba, Didace; Bilulu, Guy; Mbenzo-Abokome, Valentin; Inogwabini, Bila-Isia; Muyembe-Tamfum, Jean-Jacques; Delaporte, Eric; Peeters, Martine

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Novel Multiplexed HIV/Simian Immunodeficiency Virus Antibody Detection Assay

Published Date:

Dec 2011

Source:

Emerg Infect Dis. 17(12):2277-2286.

[PDF - 417.95 KB]

Details:

Personal Authors:

Ahuka-Mundeke, Steve ; Ayouba, Ahidjo ; Mbala-Kingebeni, Placide ; Liegeois, Florian ; Esteban, Amandine ; ... More ▼

Keywords:

[+]

Description:

Like most emerging infectious disease viruses, HIV is also of zoonotic origin. To assess the risk for cross-species transmission of simian immunodeficiency viruses (SIVs) from nonhuman primates to humans in the Democratic Republic of Congo, we collected 330 samples derived from nonhuman primate bushmeat at 3 remote forest sites. SIV prevalences were estimated by using a novel high-throughput assay that included 34 HIV and SIV antigens in a single well. Overall, 19% of nonhuman primate bushmeat was infected with SIVs, and new SIV lineages were identified. Highest SIV prevalences were seen in red-tailed guenons (25%) and Tshuapa red colobus monkeys (24%), representing the most common hunted primate species, thus increasing the likelihood for cross-species transmission. Additional studies are needed to determine whether other SIVs crossed the species barrier. With the newly developed assay, large-scale screening against many antigens is now easier and faster.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Funding:

R01 AI 50529/AI/NIAID NIH HHS/United States

Supporting Files:

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