Peak Focusing Based on Stationary Phase Thickness Gradient

Details

• Personal Author:
  Fan X ; Kurabayashi K ; Li MW-H ; Li Z ; She J ; Zhou M ; Zhu H
• Description:
  This paper reports the development of a stationary phase thickness gradient gas chromatography (GC) column that enables analyte peak focusing and improves separation resolution. Theoretical analysis and simulation demonstrate focusing via a positive thickness gradient, i.e., the stationary phase thickness increases along the column. This effect was experimentally verified by coating a 5?m long capillary column with a film thickness varying from 34?nm at the column inlet to 241?nm at the column outlet. The column was analyzed in forward (thin to thick) and backward (thick to thin) modes and compared to a uniform thickness column with a thickness of 131?nm, using alkanes ranging from C5 to C16 and aromatics. Comparison of resolutions between forward mode and the uniform thickness column demonstrated an overall focusing rate (i.e., improvement in peak capacity) of 11.7% on alkanes and 28.2% on aromatics. The focusing effect was also demonstrated for isothermal room temperature separation of highly volatile compounds and temperature programmed separation with different ramping rates. In all cases, peak capacities from forward mode separations are higher than those from other modes, indicating the ability of a positive thickness gradient to focus analyte peaks. This thickness gradient technique can therefore be broadly applied to various stationary phases and column types as a general method for improving GC separation performance. [Description provided by NIOSH]
• Subjects:
  Alkanes Chemistry, Analytic Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Instruments Author Keywords: Gas Chromatography Gas Chromatography Peak Capacity Peak Focusing Resolution Stationary Phase Gradient Thickness Gradient Volatiles
• ISSN:
  0021-9673
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  1614
• NIOSHTIC Number:
  nn:20068452
• Citation:
  J Chromatogr A 2020 Mar; 1614:460737
• Contact Point Address:
  Xudong Fan, Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
• Email:
  xsfan@umich.edu
• Federal Fiscal Year:
  2020
• Performing Organization:
  University of Michigan at Ann Arbor
• Peer Reviewed:
  True
• Start Date:
  20180901
• Source Full Name:
  Journal of Chromatography A
• End Date:
  20220831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ce396ed0044a097b18732a6be44abed1cb80dd550de7db6987fdd3eba92a6c76403f7eb78c83ca3b3870885195b3ce4d156a6b45d9cf3be60734d013d88c5a1b
• Download URL:
  https://stacks.cdc.gov/view/cdc/230605/cdc_230605_DS1.pdf
• File Type:
  [PDF - 1.98 MB ]