Au@PtPd Enhanced Immunoassay with 3D Printed Smartphone Device for Quantification of Diaminochlorotriazine (DACT), the Major Atrazine Biomarker

Details

• Personal Author:
  Cheng N ; Davis WC ; Du D ; Hulubei V ; Lin Y ; Lyu Z ; Ruan X ; Shi Q ; Smith JN ; Wang L ; Wang Y
• Description:
  Increased use of pesticides in agriculture requires new advanced techniques to monitor both environmental levels and human exposure of pesticides to avoid potential adverse health outcomes in sensitive populations. Atrazine is widely used to control broadleaf weeds, and here we developed a new sensor capable of detecting diaminochlorotriazine (DACT), the major metabolite and biomarker of atrazine exposure. We established an Au@PtPd nanoparticles labeled lateral flow immunoassay (LFIA) for immunochromatographic based rapid detection of urinary DACT. The detection was based on competitive immunoassay between the analyte and the BSA-conjugated antigen. As evaluated, the coupled mesoporous core-shell Au@PtPd nanoparticles, with superior peroxidase-like activity, as the signal indicator offers a rapid direct chromatographic readout inversely correlated with the concentration of analytes, providing a detection limit of 0.7 ng/mL for DACT. Moreover, the detection limits were boosted to as low as 11 pg/mL with the detectable range from 10 pg/ml to 10 ng/mL, through a one-step catalytic chromogenic reaction. A rapid readout device was developed by 3D printing to provide a stable real-time quantification of the color intensity capable of assessing both chromatographic and absorbance results. This Au@PtPd nanoparticle-based immunosensing platform, as well as the 3D printed readout device, provide a promising tool for on-site and ultrasensitive detection of pesticide biomarkers. [Description provided by NIOSH]
• Subjects:
  Biomarkers Energy Metabolism Insecticides Occupational Health Printing, Three-Dimensional Safety
• Keywords:
  3D Printing Atrazine Au@PtPd Nanoparticle Author Keywords: Lateral Flow Immunoassay Biological Monitoring Immunoassays Metabolites Nanoparticles Peroxidase-like Activity Peroxidases Pesticide Metabolite Pesticides Sensors Three Dimensional Printing

  More +
• ISSN:
  0956-5663
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  208
• NIOSHTIC Number:
  nn:20067933
• Citation:
  Biosens Bioelectron 2022 Jul; 208:114190
• Contact Point Address:
  Dan Du, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164-2920, United States
• Email:
  annie.du@wsu.edu
• CAS Registry Number:
  Atrazine (CAS RN 1912-24-9)
• Federal Fiscal Year:
  2022
• Performing Organization:
  Battelle Pacific Northwest Laboratories, Richland, Washington
• Peer Reviewed:
  True
• Start Date:
  20160901
• Source Full Name:
  Biosensors and Bioelectronics
• End Date:
  20200831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ee1980fccfbca573d22ff27d7bb921d72c4d9afd1e4287e8c560bf27bc29f5629fe96051a251588a720118927c2a8faa90afbf4972c81640248e87e2b691c9eb
• Download URL:
  https://stacks.cdc.gov/view/cdc/230167/cdc_230167_DS1.pdf
• File Type:
  [PDF - 5.33 MB ]