Phosphatidylserine Targets Single-Walled Carbon Nanotubes to Professional Phagocytes In Vitro and In Vivo

Details

• Alternative Title:
  PLoS ONE
• Personal Author:
  Konduru, Nagarjun V. ; Tyurina, Yulia Y. ; Feng, Weihong ; Basova, Liana V. ; Belikova, Natalia A. ; Bayir, Hülya ; Clark, Katherine ; Rubin, Marc ; Stolz, Donna ; Vallhov, Helen ; Scheynius, Annika ; Witasp, Erika ; Fadeel, Bengt ; Kichambare, Padmakar D. ; Star, Alexander ; Kisin, Elena R. ; Murray, Ashley R. ; Shvedova, Anna A. ; Kagan, Valerian E.
• Description:
  Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological action. We report that SWCNT coating with a phospholipid "eat-me" signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells.
• Subjects:
  Animals Cell Line, Tumor Female Flow Cytometry HeLa Cells Humans In Vitro Techniques Macrophages Mice Mice, Inbred C57BL Microscopy, Electron, Scanning Microscopy, Electron, Transmission Nanotubes, Carbon Phagocytes Phosphatidylserines Rats

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• Source:
  PLoS ONE. 2009; 4(2).
• Pubmed ID:
  19198650
• Pubmed Central ID:
  PMC2634966
• Document Type:
  Journal Article
• Funding:
  OH008282/OH/NIOSH CDC HHS/United States
• Volume:
  4
• Issue:
  2
• NIOSHTIC Number:
  nn:20035032
• Collection(s):
  CDC Public Access National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b2b328a8bd9f451b671765608e22dda77fbf01b7c27e63281c830dc9da18024e3a76dd4767ecbe48aa3c5184b8705b9411f28e8ecd6f7304843c4b61bd420ad7
• Download URL:
  https://stacks.cdc.gov/view/cdc/22995/cdc_22995_DS1.pdf
• File Type:
  [PDF - 2.59 MB ]