Details:

Alternative Title:
PLoS ONE
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0004398
Personal Author:
Konduru, Nagarjun V. ; Tyurina, Yulia Y. ; Feng, Weihong ; Basova, Liana V. ; Belikova, Natalia A. ; ... More +
Konduru, Nagarjun V. ; Tyurina, Yulia Y. ; Feng, Weihong ; Basova, Liana V. ; Belikova, Natalia A. ; Bayir, Hülya ; Clark, Katherine ; Rubin, Marc ; Stolz, Donna ; Vallhov, Helen ; Scheynius, Annika ; Witasp, Erika ; Fadeel, Bengt ; Kichambare, Padmakar D. ; Star, Alexander ; Kisin, Elena R. ; Murray, Ashley R. ; Shvedova, Anna A. ; Kagan, Valerian E. Less -
Description:
Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological action. We report that SWCNT coating with a phospholipid "eat-me" signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells.
Subject:
[+]
Animals
Biotechnology
Cell Line, Tumor
Female
Flow Cytometry
HeLa Cells
Humans
In Vitro Techniques
Macrophages
Mice
Mice, Inbred C57BL
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Nanotubes, Carbon
Phagocytes
Phosphatidylserines
Rats
Research Article

Source:
PLoS ONE. 2009; 4(2).
Pubmed ID:
19198650
Pubmed Central ID:
PMC2634966
Document Type:
Journal Article
Funding:
OH008282/OH/NIOSH CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d0a0cf359119daf4d9dd1ec28463ef2af0ae2e2a34777927a290c610fac11fd9
File Type:

Supporting Files

• 	pone.0004398.g004.jpg	jpeg
  	pone.0004398.g005.gif	gif
  	pone.0004398.g005.jpg	jpeg
  	pone.0004398.g006.gif	gif
  	pone.0004398.g006.jpg	jpeg
  	pone.0004398.g007.gif	gif
  	pone.0004398.g007.jpg	jpeg
  	pone.0004398.g008.gif	gif
  	pone.0004398.g008.jpg	jpeg
  	pone.0004398.nxml	txt
  	license.txt	txt
  	pone.0004398.s001.tif	tiff
  	pone.0004398.s002.tif	tiff
  	pone.0004398.s003.tif	tiff
  	pone.0004398.s004.doc	doc
  	pone.0004398.g001.gif	gif
  	pone.0004398.g001.jpg	jpeg
  	pone.0004398.g002.gif	gif
  	pone.0004398.g002.jpg	jpeg
  	pone.0004398.g003.gif	gif
  	pone.0004398.g003.jpg	jpeg
  	pone.0004398.g004.gif	gif

More +