Genetic Dissection of an Exogenously Induced Biofilm in Laboratory and Clinical Isolates of E. coli

Amini, Sasan; Goodarzi, Hani; Tavazoie, Saeed

doi:10.1371/journal.ppat.1000432

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Genetic Dissection of an Exogenously Induced Biofilm in Laboratory and Clinical Isolates of E. coli

May 15 2009
By Amini, Sasan ; Goodarzi, Hani ; Tavazoie, Saeed
http://dx.doi.org/10.1371/journal.ppat.1000432
Source: PLoS Pathog. 2009; 5(5).

[PDF-1.49 MB]

English

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Details:

Alternative Title:

PLoS Pathog
Personal Author:

Amini, Sasan ; Goodarzi, Hani ; Tavazoie, Saeed

Amini, Sasan ; Goodarzi, Hani ; Tavazoie, Saeed Less -
Description:

Microbial biofilms are a dominant feature of many human infections. However, developing effective strategies for controlling biofilms requires an understanding of the underlying biology well beyond what currently exists. Using a novel strategy, we have induced formation of a robust biofilm in Escherichia coli by utilizing an exogenous source of poly-N-acetylglucosamine (PNAG) polymer, a major virulence factor of many pathogens. Through microarray profiling of competitive selections, carried out in both transposon insertion and over-expression libraries, we have revealed the genetic basis of PNAG-based biofilm formation. Our observations reveal the dominance of electrostatic interactions between PNAG and surface structures such as lipopolysaccharides. We show that regulatory modulation of these surface structures has significant impact on biofilm formation behavior of the cell. Furthermore, the majority of clinical isolates which produced PNAG also showed the capacity to respond to the exogenously produced version of the polymer.
Subjects:

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Bacterial Proteins Beta-Glucans Biofilms Escherichia Coli Escherichia Coli Proteins Genes, Bacterial Genome, Bacterial Genomic Library Glycoside Hydrolases Oligonucleotide Array Sequence Analysis Spectrometry, Mass, Electrospray Ionization
Source:

PLoS Pathog. 2009; 5(5).
Pubmed ID:

19436718
Pubmed Central ID:

PMC2675270
Document Type:

Journal Article
Funding:

1DP10D003787-01/DP/NCCDPHP CDC HHS/United States ; DP1 OD003787/OD/NIH HHS/United States ; P50 GM071508/GM/NIGMS NIH HHS/United States ; R01 AI077562/AI/NIAID NIH HHS/United States

1DP10D003787-01/DP/NCCDPHP CDC HHS/United States ; DP1 OD003787/OD/NIH HHS/United States ; P50 GM071508/GM/NIGMS NIH HHS/United States ; R01 AI077562/AI/NIAID NIH HHS/United States Less -
Volume:

5
Issue:

5
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:d05340e36647f3cf9f9543099b5b6f505231ca233556e340591f03c17964d03c
Download URL:

https://stacks.cdc.gov/view/cdc/22983/cdc_22983_DS1.pdf
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