Global Discovery of Adaptive Mutations

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Goodarzi, Hani ; Hottes, Alison K. ; Tavazoie, Saeed
• Description:
  Although modern DNA sequencing enables rapid identification of genetic variation, characterizing the phenotypic consequences of individual mutations remains a labor-intensive task. Here we describe array-based discovery of adaptive mutations (ADAM), a technology that searches an entire bacterial genome for mutations that contribute to selectable phenotypic variation between an evolved strain and its parent. We found that ADAM identified adaptive mutations in laboratory-evolved Escherichia coli strains with high sensitivity and specificity.
• Subjects:
  Adaptation, Biological Article DNA Mutational Analysis DNA Transposable Elements Drug Resistance, Bacterial Escherichia Coli Escherichia Coli Proteins Mutation Sensitivity And Specificity
• Source:
  Nat Methods. 6(8):581-583.
• Pubmed ID:
  19597501
• Pubmed Central ID:
  PMC2762608
• Document Type:
  Journal Article
• Funding:
  1DP10D003787-01/DP/NCCDPHP CDC HHS/United States ; DP1 OD003787/OD/NIH HHS/United States ; DP1 OD003787-01/OD/NIH HHS/United States ; P50 GM071508/GM/NIGMS NIH HHS/United States ; R01 AI077562/AI/NIAID NIH HHS/United States
• Volume:
  6
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b8fa1da7974e43bf3ff85e86593c5493dd68447aff69f60f50b087f3f1c8dc68
• Download URL:
  https://stacks.cdc.gov/view/cdc/22930/cdc_22930_DS1.pdf
• File Type:
  [PDF - 1.57 MB ]