Effects of Silica Exposure on Substance P Immunoreactivity and Preprotackykinin mRNA Expression in Trigeminal Sensory Neurons

Details

• Personal Author:
  Dey RD ; Hunter DD ; Stanley CF
• Description:
  Trigeminal sensory neurons innervate the nasal cavity and may release substance P (SP) upon exposure to inhalated irritants. The purpose of this study was to determine if silica dust, an occupational irritant causing inflammation, activates sensory neurons supplying the nasal cavity. Rats were placed in inhalation chambers and exposed daily to 2mg/m3 of fresh silica (<5um) or filtered air for six months (n=3). The trigeminal ganglia (TG) were removed and prepared for SP immunocytochemistry and preprotackykinin (PPT) in situ hybridization. SP-like immunofluorescence in TG neurons was categorized as high, moderate, or low intensity. In situ hybridization autoradiographs were quantified on the basis of grain density using digital imaging analysis. The SP immunoreactivity and PPT mRNA expression in the TG neurons were significantly increased after silica inhalation. The proportion of highly positive SP-immunoreactive neurons shifted from 1.30+/-.58% in controls to 11.30+/-1.l5% after silica treatment. The neurons exhibiting high grain density increased from 1.50+/-.87% in controls to 11.67%+/-.58 in the silica group. Thus, inhalation of silica results in increased levels of immunoreactive neuronal SP and PPT mRNA. These findings suggest that silica activates sensory pathways which may be involved in nasal inflammation. [Description provided by NIOSH]
• Subjects:
  Biochemistry Environmental Monitoring Immune System Laboratories Nervous System Nervous System Diseases Nose Occupational Health Particulate Matter Respiratory System Safety Sense Organs

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• Keywords:
  Biochemical-analysis Biological-effects Biological-systems Cell-biology Cell-function Cellular-reactions Exposure-assessment Exposure-methods Immune-reaction Immune-system Immunochemistry Inhalation-studies Laboratory-animals Laboratory-techniques Laboratory-testing Molecular-biology Molecular-structure Nasal-cavity Nasal-disorders Neurovascular-disorders Particle-aerodynamics Particle-counters Particulates Quantitative-analysis

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• Series:
  Mining Publications
• ISSN:
  0892-6638
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  3
• NIOSHTIC Number:
  nn:20035888
• Citation:
  FASEB J 1995 Mar; 9(3)(I):A433
• Contact Point Address:
  Department of Anatomy and Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506
• CAS Registry Number:
  Silica (CAS RN 7631-86-9)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  False
• Part Number:
  I
• Source Full Name:
  The FASEB Journal. Experimental Biology 95 - Annual Meeting of Professional Research Scientists, Atlanta, Georgia, April 9-13, 1995
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b26bf961ddee4f17d924abc8bd9646ba2586a608aa97c9767043a64a843cd648a8aa9b8e42d224e3c13117d2347f7eae3ae07666a2f6392ea4867829584e7444
• Download URL:
  https://stacks.cdc.gov/view/cdc/227128/cdc_227128_DS1.pdf
• File Type:
  [PDF - 2.35 MB ]