Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles In Vivo

Details

• Personal Author:
  Bauer SM ; Bezemer GFG ; Breysse PN ; Georas SN ; Oberdörster G ; Pieters RHH ; Williams MA
• Description:
  The biological effects of acute particulate air pollution exposure in host innate immunity remain obscure and have relied largely on in vitro models. We hypothesized that single acute exposure to ambient or engineered particulate matter (PM) in the absence of other secondary stimuli would activate lung dendritic cells (DC) in vivo and provide information on the early immunological events of PM exposure and DC activation in a mouse model naïve to prior PM exposure. Activation of purified lung DC was studied following oropharyngeal instillation of ambient particulate matter (APM). We compared the effects of APM exposure with that of diesel-enriched PM (DEP), carbon black particles (CBP) and silver nanoparticles (AgP). We found that PM species induced variable cellular infiltration in the lungs and only APM exposure induced eosinophilic infiltration. Both APM and DEP activated pulmonary DC and promoted a Th2-type cytokine response from naïve CD4+ T cells ex vivo. Cultures of primary peribronchial lymph node cells from mice exposed to APM and DEP also displayed a Th2-type immune response ex vivo. We conclude that exposure of the lower airway to various PM species induces differential immunological responses and immunomodulation of DC subsets. Environmental APM and DEP activated DC in vivo and provoked a Th2 response ex vivo. By contrast, CBP and AgP induced altered lung tissue barrier integrity but failed to stimulate CD4+ T cells as effectively. Our work suggests that respirable pollutants activate the innate immune response with enhanced DC activation, pulmonary inflammation and Th2-immune responsiveness. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution Immune System Lung Occupational Health Particulate Matter Respiratory System Safety Toxicology Vehicle Emissions
• Keywords:
  Airborne Particles Air Pollution Allergic Immunity Author Keywords: Innate Immunity Cellular Effects Dendritic Cell Diesel Exhausts Immune Reaction Immune System Immunological Tests Immunotoxicology Inflammation In Vivo Studies Lung Tissue Nanoparticles Particulate Matter Particulates Silver

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• ISSN:
  1662-811X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; New York ; OSHA Region 2 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  150-166
• Volume:
  3
• Issue:
  2
• NIOSHTIC Number:
  nn:20062720
• Citation:
  J Innate Immun 2011 Feb; 3(2):150-166
• Contact Point Address:
  Dr. Marc Adrian Williams, NHEERL, Environmental Public Health Division, Cardiopulmonary and Immunotoxicology Branch, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27711
• Email:
  Williams.marc@epa.gov
• Federal Fiscal Year:
  2011
• Performing Organization:
  Johns Hopkins University
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Innate Immunity
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:62a4f0586b174941e98ae31237754402632d17ec45ae9d94c6ebad2d33327a6350d626fdc9ec2d1f18bbad6b2cb5bd777966b6da1c078303e95af4ccd5d6243e
• Download URL:
  https://stacks.cdc.gov/view/cdc/225912/cdc_225912_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]