Details:

Alternative Title:
Nat Biotechnol
Personal Author:
Hwang, Woong Y. ; Fu, Yanfang ; Reyon, Deepak ; Maeder, Morgan L. ; Tsai, Shengdar Q. ; ... More +
Hwang, Woong Y. ; Fu, Yanfang ; Reyon, Deepak ; Maeder, Morgan L. ; Tsai, Shengdar Q. ; Sander, Jeffry D. ; Peterson, Randall T. ; Yeh, J.-R. Joanna ; Joung, J. Keith Less -
Description:
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Subject:
[+]
Animals
Article
Base Sequence
DNA
DNA Cleavage
Embryo, Nonmammalian
Endonucleases
Genetic Engineering
Genome
Inverted Repeat Sequences
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA, Guide
RNA Editing
Zebrafish

Source:
Nat Biotechnol. 31(3):227-229.
Pubmed ID:
23360964
Pubmed Central ID:
PMC3686313
Document Type:
Journal Article
Funding:
DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; F32 GM105189/GM/NIGMS NIH HHS/United States ; K01 AG031300/AG/NIA NIH HHS/United States ; K01 AG031300/AG/NIA NIH HHS/United States ; ... More +
DP1 GM105378/DP/NCCDPHP CDC HHS/United States ; DP1 GM105378/GM/NIGMS NIH HHS/United States ; F32 GM105189/GM/NIGMS NIH HHS/United States ; K01 AG031300/AG/NIA NIH HHS/United States ; K01 AG031300/AG/NIA NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; P50 HG005550/HG/NHGRI NIH HHS/United States ; R01 CA140188/CA/NCI NIH HHS/United States ; R01 GM088040/GM/NIGMS NIH HHS/United States ; R01 GM088040/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:9108169962024660d5992e6f9b1b0286bbdb1dabec048bd9e356eb6ca80472c9
File Type:

Supporting Files

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