Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

Details

• Alternative Title:
  Nat Biotechnol
• Personal Author:
  Hwang, Woong Y. ; Fu, Yanfang ; Reyon, Deepak ; Maeder, Morgan L. ; Tsai, Shengdar Q. ; Sander, Jeffry D. ; Peterson, Randall T. ; Yeh, J.-R. Joanna ; Joung, J. Keith
• Description:
  In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
• Subjects:
  Animals Article Base Sequence CRISPR-Cas Systems DNA DNA Cleavage Embryo, Nonmammalian Endonucleases Genetic Engineering Genome Inverted Repeat Sequences Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Guide, Kinetoplastida RNA Editing Zebrafish

  More +
• Source:
  Nat Biotechnol. 31(3):227-229
• Pubmed ID:
  23360964
• Pubmed Central ID:
  PMC3686313
• Document Type:
  Journal Article
• Funding:
  DP1 GM105378/GM/NIGMS NIH HHSUnited States/ ; P50 HG005550/HG/NHGRI NIH HHSUnited States/ ; R01 CA140188/CA/NCI NIH HHSUnited States/ ; K01 AG031300/AG/NIA NIH HHSUnited States/ ; DP1 GM105378/DP/NCCDPHP CDC HHSUnited States/ ; R01 GM088040/GM/NIGMS NIH HHSUnited States/ ; F32 GM105189/GM/NIGMS NIH HHSUnited States/
• Volume:
  31
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:9108169962024660d5992e6f9b1b0286bbdb1dabec048bd9e356eb6ca80472c9
• Download URL:
  https://stacks.cdc.gov/view/cdc/22570/cdc_22570_DS1.pdf
• File Type:
  [PDF - 1.42 MB ]