Pulmonary Inflammation Response Comparison of Nano-Clay to Machined Dusts of Nano-Clay-Enabled Composite

Details

• Personal Author:
  Agarwal S ; Battelli L ; Coyle J ; Derk R ; Dinu CZ ; Friend S ; Gupta RK ; Jensen J ; Johnson C ; Lee EG ; Orandle M ; Roach K ; Rojanasakul L ; Shane H ; Stueckle, Todd A.
• Description:
  Advanced manufacturing techniques increasingly rely on engineered nanomaterials (ENMs) as filler material for nanocomposites (NCs). Organomodified nanoclays (ONCs) represent a significant proportion of ENM fillers used in NCs to enhance strength, barrier function, and flammability. One class of organic coatings, quaternary ammonium compounds, possess known inflammatory, sensitization, and cytotoxic properties following dermal and inhalation exposure. At present, a need exists to evaluate how repeated inhalation exposure to ONCs and machining dust particles released from nanoclay-enabled NCs impacts pulmonary inflammation responses. In the present study female Balb/C mice were exposed via six repeated aspirations to Cloisite 93A (an ONC; 16.7 or 41.7 microg), virgin polypropylene (VPP) NC machining dust, and 1% Cloisite 93A PP NC dust (50 or 150 microg) particles. Bronchioalveolar lavage (BAL) and lung tissue samples were collected up to 28 days post-exposure to evaluate inflammatory markers. Collected BAL fluid showed transient infiltrates of neutrophils, eosinophils, B cells, CD4+ T cells, and CD8+ T cells dose-dependently in particle-exposed mice. Neutrophil infiltrates persisted up to day 28 in Clois93A-exposed mice. Clois93A caused elevated interstitial CD4+ T cells (day 1) while NP exposed animals showed elevated interstitial B cells. Particle exposure caused transient increases in cDC2s, pDCs, interstitial macrophages, and activated (CD86+) monocytes. Clois93A induced elevated CD11b+ DCs (day 1 and 7) and CD86+ CD103+ DCs (day 1 and 28). Irrespective of ONC or particle, hyper-acute inflammatory cytokine induction was observed, characterized by acute inflammatory (MIP1a, MDC, IL1B, TNFa, IL6, CXCL1, CXCL2), Th2 (IL5, IL13), and tissue remodeling (TIMP1, MMP9) cytokines. High dose Clois93A and 1% Clois93A PP also elicited MIP1a, MDC, MMP9, CXCL2, IL1a release on day 7 and elevated MIP1a, IL5, and IL6 on day 28 compared to other treatments. In summary, ONC presence and particle source along the ONC lifecycle influenced leukocyte airway, interstitial, and inflammatory marker responses. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Dust Immune System Manufacturing And Industrial Facilities Occupational Health Particulate Matter Respiratory System Safety Toxicology
• Keywords:
  Animal Studies Dust Particles Immune Reaction Inhalation Studies Manufacturing Nanoclays Nanocomposites Nanomaterials Physiological Response
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  180
• NIOSHTIC Number:
  nn:20062266
• Citation:
  Toxicologist 2021 Mar; 180(S1):116
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 60th Annual Meeting and ToxExpo, March 12-26, 2021, Virtual Event
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bdfd47f687fed1db834b79369924615794b0b2083afb383a4a826eba8e00234aa870ff3415ea5c337e0b9c344e07239cc9edf8858e791b1a038c867f7ad62132
• Download URL:
  https://stacks.cdc.gov/view/cdc/225547/cdc_225547_DS1.pdf
• File Type:
  [PDF - 142.34 KB ]