Comparison of Samplers Collecting Airborne Influenza Viruses: 1. Primarily Impingers and Cyclones

Details

• Personal Author:
  Aboubakr HA ; Adesina A ; Goyal SM ; Raynor PC ; Torremorell M ; Yang M
• Description:
  Researchers must be able to measure concentrations, sizes, and infectivity of virus-containing particles in animal agriculture facilities to know how far infectious virus-containing particles may travel through air, where they may deposit in the human or animal respiratory tract, and the most effective ways to limit exposures to them. The objective of this study was to evaluate a variety of impinger and cyclone aerosol or bioaerosol samplers to determine approaches most suitable for detecting and measuring concentrations of virus-containing particles in air. Six impinger/cyclone air samplers, a filter-based sampler, and a cascade impactor were used in separate tests to collect artificially generated aerosols of MS2 bacteriophage and swine and avian influenza viruses. Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. The influenza viruses were titrated in cell cultures to determine quantities of infectious virus. Viral RNA was extracted and used for quantitative real time RT-PCR, to provide total virus concentrations for all three viruses. The amounts of virus recovered and the measured airborne virus concentrations were calculated and compared among the samplers. Not surprisingly, high flow rate samplers generally collected greater quantities of virus than low flow samplers. However, low flow rate samplers generally measured higher, and likely more accurate, airborne concentrations of Infectious virus and viral RNA than high flow samplers. To assess airborne viruses in the field, a two-sampler approach may work well. A suitable high flow sampler may provide low limits of detection to determine if any virus is present in the air. If virus is detected, a suitable lower flow sampler may measure airborne virus concentrations accurately. [Description provided by NIOSH]
• Subjects:
  Agriculture Air Pollutants, Occupational Communicable Diseases Environmental Monitoring Influenza, Human Livestock Occupational Health Safety Viral Diseases
• Keywords:
  Agricultural Industry Airborne Particles Air Sampling Animal Handlers Influenza Sampling Equipment Viral Diseases
• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  1
• NIOSHTIC Number:
  nn:20062100
• Citation:
  PLoS One 2021 Jan; 16(1):e0244977
• Contact Point Address:
  Peter C. Raynor, Division of Environmental Health Sciences, University of Minnesota, School of Public Health, Minneapolis, Minnesota, United States of America
• Email:
  praynor@umn.edu
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Minnesota
• Peer Reviewed:
  True
• Start Date:
  20110930
• Source Full Name:
  PLoS One
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b047541abd1426bdc951e9649bbce5674d3cc4a3a293c2c9e279077de48078bb5b8b99f328d1eef6980d549cf0211a8ad4196214ccb51772256a7d3441658868
• Download URL:
  https://stacks.cdc.gov/view/cdc/225413/cdc_225413_DS1.pdf
• File Type:
  [PDF - 2.75 MB ]