Pre-Exposure to Hydrogen Sulfide Modulates the Innate Inflammatory Response to Organic Dust

Details

• Personal Author:
  Bhat SM ; Charavaryamath C ; Massey N ; Rumbeiha WK ; Santana Maldonado C ; Shrestha D
• Description:
  Animal production units produce and store many contaminants on-site, including organic dust (OD) and hydrogen sulfide (H2S). Workers in these settings report various respiratory disease symptoms. Both OD and H2S have shown to induce lung inflammation. However, impact of co-exposure to both H2S and OD has not been investigated. Therefore, we tested a hypothesis that pre-exposure to H2S modulates the innate inflammatory response of the lungs to organic dust. In a mouse model of H2S and organic dust extract (ODE) exposure, we assessed lung inflammation quantitatively. We exposed human airway epithelial and monocytic cells to medium or H2S alone or H2S followed by ODE and measured cell viability, oxidative stress, and other markers of inflammation. Exposure to 10 ppm H2S followed by ODE increased the lavage fluid leukocytes. However, exposure to 10 ppm H2S alone resulted in changes in tight junction proteins, an increase in mRNA levels of tlr2 and tlr4 as well as ncf1, ncf4, hif1a, and nrf2. H2S alone or H2S and ODE exposure decreased cell viability and increased reactive nitrogen species production. ODE exposure increased the transcripts of tlr2 and tlr4 in both in vitro and in vivo models, whereas increased nfkbp65 transcripts following exposure to ODE and H2S was seen only in in vitro model. H2S alone and H2S followed by ODE exposure increased the levels of IL-1B. We conclude that pre-exposure to H2S modulates lung innate inflammatory response to ODE. [Description provided by NIOSH]
• Subjects:
  Animals Dust Livestock Lung Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Airway Epithelium Cellular Effects Hydrogen Sulfide Inflammation Lung Irritants Organic Dust Organic Dusts Oxidative Stress Proteins Tissue Culture
• ISSN:
  0302-766X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Iowa ; OSHA Region 7 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  129-148
• Volume:
  384
• Issue:
  1
• NIOSHTIC Number:
  nn:20061801
• Citation:
  Cell Tissue Res 2021 Apr; 384(1):129-148
• Contact Point Address:
  Chandrashekhar Charavaryamath, Biomedical Sciences, Iowa State University, Ames, IA 50011, USA
• Email:
  chandru@iastate.edu
• CAS Registry Number:
  Hydrogen sulfide (CAS RN 7783-06-4)
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Iowa, Iowa City
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Cell and Tissue Research
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:42db042cc04f25499b066639d4deaff5ab69bebf289db5a16b90bd1905ec2818f29d02b3b297d30767114f434cb3229c8d690a3375f5fcdfdb958f066ff63ef2
• Download URL:
  https://stacks.cdc.gov/view/cdc/225181/cdc_225181_DS1.pdf
• File Type:
  [PDF - 1.15 MB ]