Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers

Ahmed A; Aleckovic M; Ambati SR; Ararso Y; Bailey KA; Balachandran VP; Basu EM; Batra SK; Benito-Martin A; Berger M; Bojmar L; Brady MS; Buehring W; Cardoso F; Chou AJ; Cioffi M; Danzer E; Davies AE; De Braganca KC; DeStefano J; Di Giannatale A; Diolaiti D; Donzelli M; Fischer C; Freitas D; Ganshaw L; García-Santos G; Ghajar CM; Gyan KE; Harris A; Hashimoto A; Heissel S; Hernandez J; Hoshino A; Jain M; Jolissant JS; Kenific CM; Khakoo Y; Kim HS; Krantz BA; Lacayo NJ; Lauritzen P; Lucotti S; Malladi M; Mark MT; Marrano M; Meyers PA; Modak S; Molina H; Nakajima M; Nogués L; Offer K; Ogitani Y; Oren M; Pelissier Vatter FA; Petriccione M; Posner Z; Raju GP; Roberts SS; Rodrigues G; Roehrl MHA; Rudin CM; Sagi I; Schaeffer LM; Scherz A; Scherz-Shouval R; Simeone DM; Simpson AL; Slotkin EK; Stanger BZ; Steiner L; Sugiura K; Takahashi N; Vincent TC; Vitolano S; Wang H; Weiser MR; Wexler LH; Williams C; Wright GP; Yarden Y; Zambirinis CP

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Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers

2020/08/20
By Ahmed A ; Aleckovic M ; Ambati SR ; ...

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Personal Author:

Ahmed A ; Aleckovic M ; Ambati SR ; Ararso Y ; Bailey KA ; Balachandran VP ; Basu EM ; Batra SK ; Benito-Martin A ; Berger M ; Bojmar L ; Brady MS ; Buehring W ; Cardoso F ; Chou AJ ; Cioffi M ; Danzer E ; Davies AE ; De Braganca KC ; DeStefano J ; Di Giannatale A ; Diolaiti D ; Donzelli M ; Fischer C ; Freitas D ; Ganshaw L ; García-Santos G ; Ghajar CM ; Gyan KE ; Harris A ; Hashimoto A ; Heissel S ; Hernandez J ; Hoshino A ; Jain M ; Jolissant JS ; Kenific CM ; Khakoo Y ; Kim HS ; Krantz BA ; Lacayo NJ ; Lauritzen P ; Lucotti S ; Malladi M ; Mark MT ; Marrano M ; Meyers PA ; Modak S ; Molina H ; Nakajima M ; Nogués L ; Offer K ; Ogitani Y ; Oren M ; Pelissier Vatter FA ; Petriccione M ; Posner Z ; Raju GP ; Roberts SS ; Rodrigues G ; Roehrl MHA ; Rudin CM ; Sagi I ; Schaeffer LM ; Scherz A ; Scherz-Shouval R ; Simeone DM ; Simpson AL ; Slotkin EK ; Stanger BZ ; Steiner L ; Sugiura K ; Takahashi N ; Vincent TC ; Vitolano S ; Wang H ; Weiser MR ; Wexler LH ; Williams C ; Wright GP ; Yarden Y ; Zambirinis CP
Description:

There is an unmet clinical need for improved tissue and liquid biopsy tools for cancer detection. We investigated the proteomic profile of extracellular vesicles and particles (EVPs) in 426 human samples from tissue explants (TEs), plasma, and other bodily fluids. Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers. To confirm that EVPs are ideal diagnostic tools, we analyzed proteomes of TE- (n = 151) and plasma-derived (n = 120) EVPs. Comparison of TE EVPs identified proteins (e.g., VCAN, TNC, and THBS2) that distinguish tumors from normal tissues with 90% sensitivity/94% specificity. Machine-learning classification of plasma-derived EVP cargo, including immunoglobulins, revealed 95% sensitivity/90% specificity in detecting cancer. Finally, we defined a panel of tumor-type-specific EVP proteins in TEs and plasma, which can classify tumors of unknown primary origin. Thus, EVP proteins can serve as reliable biomarkers for cancer detection and determining cancer type. [Description provided by NIOSH]
Subjects:

Biomarkers Diagnosis Neoplasms Occupational Health Safety
Keywords:

Author Keywords: Exosomesexomeres Biological Monitoring Cancer Cancer Of Unknown Primary Origin Clinical Diagnosis Damage-associated Molecular Patterns Diagnostic Techniques Early Cancer Detection Extracellular Vesicles And Particles Humans Liquid Biopsy Proteins Proteomics Tumors
ISSN:

0092-8674
Document Type:

Text
Funding:

Cooperative Agreement
Genre:

Journal Article
Place as Subject:

California ; Maryland ; Nebraska ; New Jersey ; New York ; Ohio ; OSHA Region 1 ; OSHA Region 10 ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 7 ; OSHA Region 9 ; Pennsylvania ; Vermont ; Washington
CIO:

National Institute for Occupational Safety and Health (NIOSH)
Topic:

Workplace Safety & Health
Location:

North America
Volume:

182
Issue:

4
NIOSHTIC Number:

nn:20061652
Citation:

Cell 2020 Aug; 182(4):1044-1061.e18
Contact Point Address:

Ayuko Hoshino, Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA
Email:

ayukohoshino@bio.titech.ac.jp
Federal Fiscal Year:

2020
NORA Priority Area:

Construction ; Manufacturing
Performing Organization:

University of Pittsburgh at Pittsburgh
Peer Reviewed:

True
Start Date:

20060901
Source Full Name:

Cell
End Date:

20260831
Collection(s):

National Institute for Occupational Safety and Health
Main Document Checksum:

urn:sha-512:cd7eee5f54ad49257e03f432f4f7e106448aff0c3e823c4878af0262571c724abc6b3ff618ac353e6de69883b44f233d51e9913d0f6bb2e08f283784190f97f4
Download URL:

https://stacks.cdc.gov/view/cdc/225113/cdc_225113_DS1.pdf
File Type:

[PDF - 1.61 MB ]

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