The Guinea Pig Model of Diisocyanate Sensitization: I. Immunologic Studies

Details

• Personal Author:
  Bernstein IL ; Chen SE
• Description:
  Two strains of guinea pigs were parenterally immunized with well-characterized diisocyanate-protein conjugates. Hapten-specific IgE antibodies were detected in the sera of English short-hair strain guinea pigs immunized with either toluene diisocyanate-human serum albumin (TDI-HSA) or hexamethylene diisocyanate-HSA (HDI-HSA) when these sera were analyzed by the 168 hr passive cutaneous anaphylaxis (PCA) technique followed by intravenous challenges with conjugates of respective ligands coupled to an unrelated carrier protein, transferrin. IgG1 antibodies and precipitating antibodies were demonstrated in Hartley strain guinea pigs immunized with TDI/HDI-HSA conjugates. The hapten specificity of these antibodies was proved by PCA inhibition experiments and antibody absorption experiments. In the precipitating antibody system, this was further confirmed by immunoelectrophoretic analysis. Cross-reactivity between HDI and TDI was not observed in the PCA experiments. However, apparent cross-reactivity in the double gel diffusion experiments was due to new antigenic determinants formed by isocyanates after conjugation with proteins. It was therefore apparent that immune responses of guinea pigs immunized with protein conjugates of bifunctional isocyanates were heterogeneous and involved multiple specificities for hapten, carrier protein, and new antigenic determinants. It was postulated that the complex nature of the immune response generated by diisocyanate compounds in the guinea pig may also serve as a more appropriate model of isocyanate-induced human sensitivity reactions, which are known to involve diverse immunologic and nonimmunologic mechanisms. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Immune System Isocyanates Occupational Health Safety
• Keywords:
  Animal Studies Diisocyanates Hexamethylene Diisocyanate Immune Reaction Immunological Tests Proteins Sensitivity Testing
• ISSN:
  0091-6749
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  383-392
• Volume:
  70
• Issue:
  5
• NIOSHTIC Number:
  nn:20061063
• Citation:
  J Allergy Clin Immunol 1982 Nov; 70(5):383-392
• Contact Point Address:
  I. Leonard Bernstein, M.D., University of Cincinnati Medical Center, 231 Bethesda Ave., Cincinnati, OH 45267
• CAS Registry Number:
  Hexamethylene diisocyanate (CAS RN 822-06-0) ; Toluene diisocyanate (CAS RN 26471-62-5)
• Federal Fiscal Year:
  1983
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19780701
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• End Date:
  19810630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:395e7c1ffeebdce71e2a949610f65318eee7e0709bcfd3b1315945aa60b41894f0ca864cf4006f5053f247a3819e58044e7aaaa4719fd367ad28cb5ebf3dea3a
• Download URL:
  https://stacks.cdc.gov/view/cdc/224741/cdc_224741_DS1.pdf
• File Type:
  [PDF - 1.70 MB ]