Human Sperm Penetration of Zona-Free Hamster Eggs After Storage of the Semen for 48 Hours at 2 Degrees C to 5 Degrees C

Details

• Personal Author:
  Bolanos JR ; Katz DF ; Overstreet JW
• Description:
  The motility of human spermatozoa and their ability to penetrate zona-free hamster eggs were examined after dilution of the semen with TES-Tris (TEST) yolk buffer and storage for 48 hours at 2 degrees C to 5 degrees C. Semen samples from 10 fertile donors and 19 infertility patients were studied. More than 65% of the spermatozoa which were initially motile in the TEST yolk buffer remained active after storage. During storage, the mean swimming speed of the sperm declined to approximately 60% of the prestorage value. The percentage of zona-free hamster eggs that were penetrated by spermatozoa from patients and donors increased significantly following 48 hours of storage at 2 degrees C to 5 degrees C. Normal semen and abnormal semen were equally preserved by this storage method. This procedure may be used to ship semen samples by commercial transportation to specialized laboratories for testing. Low temperature storage in the TEST yolk buffer appears to enhance the fertilizing capacity of human spermatozoa in vitro. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Fertility Humans Laboratory Testing Reproductive System Disorders Spermatozoa
• ISSN:
  0015-0282
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  39
• Issue:
  4
• NIOSHTIC Number:
  nn:20060787
• Citation:
  Fertil Steril 1983 Apr; 39(4):536-541
• Contact Point Address:
  James W. Overstreet, M.D., Ph.D., Departments of Human Anatomy and Obstetrics and Gynecology, University of California, Davis, School of Medicine, Davis, California 95616
• Federal Fiscal Year:
  1983
• Performing Organization:
  University of California Davis, Davis, California
• Peer Reviewed:
  True
• Start Date:
  19820401
• Source Full Name:
  Fertility and Sterility
• End Date:
  19840331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:19b57f2d78ac614ebd10da0f6d05a3f451eba05cc234d19ea85422300a2d4d8e75304dbc21b582d6deca5816d799c5893db18008ac67a1e69ad5a5ae6a18df5c
• Download URL:
  https://stacks.cdc.gov/view/cdc/224601/cdc_224601_DS1.pdf
• File Type:
  [PDF - 693.21 KB ]