Dimensional Assessment of X-Bearing and Y-Bearing Haploid and Disomic Human Sperm with the Use of Fluorescence In Situ Hybridization and Objective Morphometry

Details

• Personal Author:
  Celik-Ozenci C ; Huszar G ; Jakab A ; Ovari L ; Sati GL ; Ward DC ; Zavaczki Z
• Description:
  To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa. Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situ hybridization. Academic clinical and research andrology laboratory. Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length. We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or the decondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid and disomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa. There are no differences, or distinguishing characteristics, in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Safety Spermatogenesis Urogenital Diseases Urogenital System
• Keywords:
  Fertility Laboratory-testing Sexual-reproduction Spermatozoa Sterility
• ISSN:
  0015-0282
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Nevada ; OSHA Region 1 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  121-127
• Volume:
  85
• Issue:
  1
• NIOSHTIC Number:
  nn:20029236
• Citation:
  Fertil Steril 2006 Jan; 85(1):121-127
• Email:
  gabor.huszar@yale.edu
• Federal Fiscal Year:
  2006
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Fertility and Sterility
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:07575dac5434e8f43a97d1a7844f6af16af1537aba87821570b4ee85a3916e638410f452a4adb373106725b40e48ffc71e15cbbea881a367395e2647a2c13f1c
• Download URL:
  https://stacks.cdc.gov/view/cdc/190269/cdc_190269_DS1.pdf
• File Type:
  [PDF - 206.19 KB ]