Leukotriene B4 Metabolism by Hepatic Cytochrome P-450

Details

• Personal Author:
  Bösterling B ; Trudell JR
• Description:
  Leukotriene B4 (LTB) was found to be metabolized by suspensions of rat liver microsomes in the presence of NADPH and oxygen. The rate of LTB metabolism was also measured in reconstituted systems of both micelles and phospholipid vesicles containing cytochrome P-450-LM2, NADPH cytochrome P-450 reductase, and cytochrome b5. A 1 uM concentration of LTB was metabolized by rat hepatic microsomes at a rate of 4 pmol LTB/min/nmole P-450, and by vesicle and micelle reconstituted systems at 3 pmole/min/nmole P-450-LM2. At this rate a 10 g rat liver exposed to 1 uM LTB can metabolize 30 ug per hour. In that the leukotrienes are pharmacologically active at nanomolar concentrations, hepatic metabolism may be an important pathway of leukotriene inactivation. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Energy Metabolism Histocytochemistry Occupational Health Safety
• Keywords:
  Animal Studies Biochemical Analysis Cytochemistry Leukocytes Liver Function Metabolism
• ISSN:
  0006-291X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  114
• Issue:
  2
• NIOSHTIC Number:
  nn:20060750
• Citation:
  Biochem Biophys Res Commun 1983 Jul; 114(2):850-854
• CAS Registry Number:
  7112-Mercaptoethanol (CAS RN 60-24-2)
• Federal Fiscal Year:
  1983
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Biochemical and Biophysical Research Communications
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:46e1807b8daa7d1d2b22c1c340bcd85ecc469645000682b0821fdaeda36090027d567b22a30c314ca900fc2e32708024703482c954f884c1068d1333ea9e9dbf
• Download URL:
  https://stacks.cdc.gov/view/cdc/224573/cdc_224573_DS1.pdf
• File Type:
  [PDF - 315.61 KB ]