Circadian Dependent Effect of Epidermal Growth Factor, Insulin and Glucagon on Hepatic Pyruvate Kinase and Malic Enzyme of Mice

Details

• Personal Author:
  Casciano DA ; Delongchamp RR ; Feuers RJ ; Pauly JE ; Scheving LA ; Scheving LE ; Tsai TH
• Description:
  The influence of epidermal growth factor (EGF), 0.75 ug g-1; insulin, 1.5 ug g-1; glucagon, 1.25 ygg-1 and their combinations on the activities of hepatic pyruvate kinase (PK) and malic enzymes (ME) was monitored. Male CD2F1 mice were treated toward the end of the light or dark periods, 9 or 23 /tours after lights on (9 or 23 HALO), and subgroups of six mice were killed at 4,8 or 12 hr post-treatment. PK and ME activities from control mice were well characterized by cosine curves. The PK activity was maximal when ME activity was minimal at the transition from light to dark (9 HALO plus 4 hr) and PK was at a minimum when ME was highest (23 HALO plus 4 hr). Both enzymes were influenced by at least one peptide hormone, and the effects were strongly circadian -stage dependent. The only effect attributed to EGF was an increase of PK activity (23%) 12 hr after injection at 23 HALO. PK activity was increased by insulin (23%) at 23 HALO (4 hr after injection), but not at 9 HALO, and decreased (17%) by glucagon 12 hr after injection at 9 HALO. Several reductions in PK activity in response to various combinations of peptides were observed, and appeared to be caused by glucagon but influenced by insulin. The activity of ME was decreased (33%) in response to insulin 4 hr after injection at 23 HALO but not at 9 HALO and increased (60-70%) by glucagon alone or in combinations with insulin or EGF, or both, at 4 hr after injection at 9 HALO but not at 23 HALO. In general, when ME activity was altered by either insulin or glucagon, PK activity was also altered in the opposite direction, and the effects of glucagon were opposed by insulin. [Description provided by NIOSH]
• Subjects:
  Animals Circadian Rhythm Enzymes Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Pyruvate Kinase Biological Rhythms Brain Circadian Circadian Rhythms Epidermal Growth Factor Glucagon Hormone Activity Insulin Liver Malic Enzyme Physiological Effects

  More +
• ISSN:
  0742-0528
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  279-286
• Volume:
  1
• Issue:
  4
• NIOSHTIC Number:
  nn:20060675
• Citation:
  Chronobiol Int 1984 Oct; 1(4):279-286
• Federal Fiscal Year:
  1985
• Performing Organization:
  University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
• Peer Reviewed:
  True
• Start Date:
  19791201
• Source Full Name:
  Chronobiology International
• End Date:
  19880301
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:75780649fc0fe5494076d1256fc28816083d632833b29d6f2e79f9950633ab30565409e06333bb6b86f8a17ed1d8976c9e99469791ca60b12cd81624ca87c9f4
• Download URL:
  https://stacks.cdc.gov/view/cdc/224516/cdc_224516_DS1.pdf
• File Type:
  [PDF - 792.49 KB ]