Action of Acrylamide on Selected Enzymes of Energy Metabolism in Denervated Cat Peripheral Nerves

Details

• Personal Author:
  Ross SM ; Sabri MI ; Spencer PS
• Description:
  The effect of acrylamide on selected glycolytic and citric acid cycle enzymes has been studied in denervated cat sciatic nerves in vitro and in vivo. The enzyme activity of glyceraldehyde-3-phosphate dehydrogenase (GADPH), neuron specific enolase (NSE), succinate dehydrogenase (SDH), and lactate dehydrogenase (LDH), has been examined in saline-perfused, desheathed and denervated peroneal (P) and tibial (T) nerves from cats treated with acrylamide (15 mg/kg/day, s.c.) or vehicle for 15 days. GAPDH activity in Schwann cells in denervated P and T nerve stumps was 2.0- and 2.3-fold higher than normal P and T nerve values. GAPDH activity in Schwann cells in denervated P and T nerves of acrylamide-treated cats was markedly reduced (56% and 61% of untreated denervated nerves, respectively). LDH and SDH activities were unaffected by acrylamide and NSE activity was absent in denervated nerve stumps. Acrylamide (0.5 and 20 mM) inhibited GAPDH activity in denervated nerve homogenates by 67% and 29%, respectively. This study demonstrates that acrylamide inhibits GAPDH in Schwann cells. The significance of GAPDH inhibition by acrylamide in denervated nerves and its relation to distal axonopathy has been discussed. [Description provided by NIOSH]
• Subjects:
  Animals Energy Metabolism Enzymes Nervous System Occupational Health Safety
• Keywords:
  Acrylamide Animal Studies Author Keywords: Acrylamide Cat Nerve Denervation Enzyme Activity Glyceraldehyde-3-phosphate Dehydrogenase Metabolic Effects Nerves Nervous System
• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  189-191
• Volume:
  340
• Issue:
  1
• NIOSHTIC Number:
  nn:20060468
• Citation:
  Brain Res 1985 Aug; 340(1):189-191
• Contact Point Address:
  S.M. Ross, Institute of Neurotoxicology, Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, U.S.A.
• CAS Registry Number:
  Acrylamide (CAS RN 79-06-1)
• Federal Fiscal Year:
  1985
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19800501
• Source Full Name:
  Brain Research
• End Date:
  19890430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:20285f05608dfa789d515ae998562d70932e36d87e85ad9adddbf51e022ed565c0dde521155109b1cb160d902e0d618262464c2ada9ce4f80e142a1438cdcf6e
• Download URL:
  https://stacks.cdc.gov/view/cdc/224346/cdc_224346_DS1.pdf
• File Type:
  [PDF - 267.51 KB ]