The Separation and Identification of Enolase Isozymes of Brain and Sciatic Nerve by High-Pressure Liquid Anion-Exchange Chromatography

Details

• Personal Author:
  Miller MS ; Sabri MI ; Soiefer AI ; Spencer PS
• Description:
  The effect of single and repeated doses of acrylamide (a neurotoxin) and N,N'-methylene-bis-acrylamide (a non-neurotoxic analogue of acrylamide) on glutathione (GSH), glutathione-S-transferase (GST) and dopamine receptors has been studied in rat brain. In vitro, both acrylamide and bis-acrylamide decreased brain GSH content in a concentration-dependent manner. At equimillimolar concentrations (2-10 mM) bis-acrylamide was more effective than acrylamide in lowering GSH levels. In vitro, GST activity was also inhibited as a function of acrylamide concentration. A single dose of either acrylamide or bis-acrylamide depleted GSH content of rat brain in a concentration-dependent manner without inhibiting GST activity. Repeated administration of either acrylamide or bis-acrylamide in rats (50 mg/kg × 10 days) decreased GSH content in the brain but GST activity was inhibited only by acrylamide and not by bis-acrylamide. Single or repeated injections of acrylamide but not of bis-acrylamide increased brain dopamine receptors (3H spiroperidol binding) in a concentration-dependent manner. [Description provided by NIOSH]
• Subjects:
  Animals Chromatography Enzymes Nervous System Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Neuron-specific Enolase Chromatographic Analysis Enzyme Activity High-pressure Liquid Chromatography Liquid Chromatography Nerves Nervous System Rodent Brain And Sciatic Nerve
• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  196-199
• Volume:
  342
• Issue:
  1
• NIOSHTIC Number:
  nn:20060466
• Citation:
  Brain Res 1985 Sep; 342(1):196-199
• Contact Point Address:
  A.I. Soifer, Institute of Neurotoxicology, Albert Einstein College of Medicine, 1300 Morris Park Ave. Kennedy Center, Rm. 522, Bronx, NY 10461, U.S.A.
• Federal Fiscal Year:
  1985
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19800501
• Source Full Name:
  Brain Research
• End Date:
  19890430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7e6a40d39a7a55090c699d17801dc75b8775ea3cf700fb5fcc028d8df0f4c5468925a51e095a726624aef5497d461b0b2bf6912f031ea11c1782a1bd3dfa8a0a
• Download URL:
  https://stacks.cdc.gov/view/cdc/224344/cdc_224344_DS1.pdf
• File Type:
  [PDF - 302.24 KB ]