Evidence for Environmental-Human Microbiota Transfer at a Manufacturing Facility with Novel Work-Related Respiratory Disease

Details

• Personal Author:
  Abraham JL ; Bachelder VD ; Blaser MJ ; Borbet TC ; Boylstein, Randy J. ; Clemente JC ; Coffre M ; Colby TV ; Cox-Ganser, Jean M. ; Crawford JA ; Cummings, Kristin J. ; Duchaine C ; Edwards, Nicole T. ; Franko AD ; Gao Z ; Green FHY ; Heguy A ; Kapoor B ; Koralov SB ; Kreiss, Kathleen ; Li Y ; Lundeen S ; Meyn P ; Nett, Randall J. ; Park, Ju-Hyeong ; Sanyal S ; Segal LN ; Stanton, Marcia L. ; Sulaiman I ; Tallaksen RJ ; Veillette M ; Virji, M. Abbas ; Warren K ; Wendland D ; Wu BG
• Description:
  Introduction: Workers' exposure to metalworking fluid (MWF) has been associated with respiratory disease. As part of a public health investigation of a manufacturing facility, we performed paired environmental and human sampling to evaluate cross-pollination of microbes between environment and host and possible effects on lung pathology present among workers. Methods: Workplace environmental microbiota was evaluated in air and MWF samples. Human microbiota was evaluated in lung tissue samples from workers with respiratory symptoms found to have lymphocytic bronchiolitis and alveolar ductitis with B-cell follicles and emphysema, lung tissue controls, and in skin, nasal and oral samples from 302 workers from different areas of the facility. In vitro effects of MWF exposure on murine B-cells were assessed. Results: Increased similarity of microbial composition was found between MWF samples and lung tissue samples of case workers compared to controls. Among workers in different locations within the facility, those that worked in machine shop area had skin, nasal and oral microbiota more closely related to the microbiota present in MWF samples. Lung samples from four index cases, and skin and nasal samples from workers in machine shop area were enriched with Pseudomonas, the dominant taxa in MWF. Exposure to used MWF stimulated murine B-cell proliferation in vitro, a hallmark cell subtype found in pathology of index cases. Conclusions: Evaluation of a manufacturing facility with a cluster of workers with respiratory disease supports cross-pollination of microbes from MWF to humans and suggests the potential for exposure to these microbes to be a health hazard. [Description provided by NIOSH]
• Subjects:
  Lung Manufacturing And Industrial Facilities Microbiology Occupational Health Pathology Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Occupational Disease Bacteria Bioaerosols Environmental Microbiota Inflammation In Vitro Study Lung Tissue Machine Shop Workers Manufacturing Metalworking Fluids Microbiome Microbiota Microorganisms MWFs Occupational Exposure Respiratory Diseases

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• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Arizona ; Minnesota ; New Jersey ; New York ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  202
• Issue:
  12
• NIOSHTIC Number:
  nn:20060457
• Citation:
  Am J Respir Crit Care Med 2020 Dec; 202(12):1678-1688
• Contact Point Address:
  Leopoldo N. Segal, New York University, Pulmonary and Critical Care Medicine, New York, New York, United States
• Email:
  leopoldo.segal@nyumc.org
• Federal Fiscal Year:
  2021
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0eb076f6450a27b694bf6b8ca342f055b6354ca8c899ca03d06db808782496c4a056e9bfd5d08f334ac787a3fb397eac90b7b0cdec669bcdce628c186ab7ee10
• Download URL:
  https://stacks.cdc.gov/view/cdc/224335/cdc_224335_DS1.pdf
• File Type:
  [PDF - 4.04 MB ]