Characterization of Microbial Components Present in Bioconcept Metalworking Fluid That May Contribute to the Development of Severe Lung Pathology

Details

• Personal Author:
  Croston T ; Lemons, Angela R. ; Stanton M ; Sulyak M
• Description:
  Rationale: Workers at a manufacturing facility using bioconcept metalworking fluid (MWF) engineered to selectively grow the biocontrol organism Pseudomonas pseudoalcaligenes developed a severe lung disease described as bronchiolitis, alveolar ductitis, and emphysema (BADE). Analysis of lung biopsies from affected workers indicated the lung microbiome was similar to that observed in used MWF. We hypothesized that this shift in the lung microbiome may contribute to the development of BADE. Methods: Pseudomonas species within used bioconcept MWF from the facility were isolated by viable culture. Bacterial 16S rRNA sequencing was conducted to ensure isolation of a single organism and to compare to P. pseudoalcaligenes strains (ATCC#17443 and ATCC#49536). Further characterization included quantification of microbial metabolites produced by the Pseudomonas isolates by liquid chromatography-tandem mass spectrometry. Results: Bacterial diversity within used MWF samples showed Pseudomonas sp. comprised 53.85% (Range: 0.07%-99.87%) of the total bacterial sequences. Two isolates were cultured and sequenced along with two P. pseudoalcaligenes strains. The 16S sequences of the isolates and ATCC strains clustered separately but were 96.6% similar, both matching deposited sequences for Pseudomonas sp. Microbial metabolites identified in all four Pseudomonas cultures included maculosin (11.2-15.4 µg/mL), cyclo(L-Pro-L-Val) (2.06-3.07 µg/mL), and brevianamide F (61.5-106 ng/mL), all of which are diketopiperazines. Conclusions: Diketopiperazines derived from microbes have known antimicrobial properties, many of which have been shown to modulate microbiome environments. High concentrations of diketopiperazines produced by Pseudomonas sp. may contribute to microbiome dysbiosis within the lungs of affected workers, which in turn may contribute to unique lung pathologies not seen in previous MWF exposures. [Description provided by NIOSH]
• Subjects:
  Lung Diseases Occupational Health Pathology Respiratory Tract Diseases Safety
• Keywords:
  Bacteria Bronchiolitis Lung Disease Metalworking Fluids MWFs
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  153
• Issue:
  2
• NIOSHTIC Number:
  nn:20070242
• Citation:
  J Allergy Clin Immunol 2024 Feb; 153(2)(Suppl):AB246
• Federal Fiscal Year:
  2024
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6b9cc76f30769b99cf667f74e9d9415a88a4997cdde82966b3547aeb96caa570aae3a41e3efc1ab33d8f7244dad52e1bf7490e46e13672b41753ff5e54842b3c
• Download URL:
  https://stacks.cdc.gov/view/cdc/208491/cdc_208491_DS1.pdf
• File Type:
  [PDF - 48.60 KB ]