Characteristics of Site Variation Among Clones of the 340-Base Pair, Tandemly Repeated EcoR1 Family of Human DNA

Details

• Personal Author:
  Flook B ; Furlong NB ; Marien K ; White J
• Description:
  Twenty-four clones of EcoR1-restricted, 340-base pair (bp) DNA derived from human DNA have been sequenced and compared to a published consensus sequence for this family. No two clones were found to have identical sequences; the clones studied differed from the consensus sequence in as few as 1 or as many as 41 sites. On the average in these clones, a 5.2% divergence from exact homology was found, with 1 of 10 of the site variations being "nonrandom," i.e., cases in which five or more clones had the same nucleotide substitution at that site (viz., 53, 124, 126, 138, 152, and 157). At site 157, for example, 16 of the 24 clones differed from the reference sequence. Positions and their respective changes, as compared to the consensus sequence, are summarized. Variations are discussed with relation to possible functions for these sequences. [Description provided by NIOSH]
• Subjects:
  Genetics Occupational Health Safety
• Keywords:
  Alphoid Sequence Author Keywords: Human DNA Deoxyribonucleic Acids DNA EcoR1 Restriction Genes Humans Nucleosomes Tandem Repeats
• ISSN:
  0006-2928
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  71-78
• Volume:
  24
• Issue:
  1
• NIOSHTIC Number:
  nn:20060330
• Citation:
  Biochem Genet 1986 Feb; 24(1-2):71-78
• Federal Fiscal Year:
  1986
• Performing Organization:
  University of Texas Hlth Sci Ctr Houston, Houston, Texas
• Peer Reviewed:
  True
• Start Date:
  19840928
• Source Full Name:
  Biochemical Genetics
• End Date:
  19880229
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9cf2116c852004182f04271843591cdb4c80ed21fa9870106f673097bcfb6a0749ad3671220d85f7cabc3f5a4ce3e3d61156a999d5094f3ba3430c8384acba16
• Download URL:
  https://stacks.cdc.gov/view/cdc/224272/cdc_224272_DS1.pdf
• File Type:
  [PDF - 401.22 KB ]