Effect of Single and Repeated Doses of Acrylamide and Bis-Acrylamide on Glutathione-S-Transferase and Dopamine Receptors in Rat Brain

Details

• Personal Author:
  Agrawal AK ; Sabri MI ; Seth PK ; Srivastava S
• Description:
  The effect of single and repeated doses of acrylamide (a neurotoxin) and N,N'-methylene-bis-acrylamide (a non-neurotoxic analogue of acrylamide) on glutathione (GSH), glutathione-S-transferase (GST) and dopamine receptors has been studied in rat brain. In vitro, both acrylamide and bis-acrylamide decreased brain GSH content in a concentration-dependent manner. At equimillimolar concentrations (2-10 mM) bis-acrylamide was more effective than acrylamide in lowering GSH levels. In vitro, GST activity was also inhibited as a function of acrylamide concentration. A single dose of either acrylamide or bis-acrylamide depleted GSH content of rat brain in a concentration-dependent manner without inhibiting GST activity. Repeated administration of either acrylamide or bis-acrylamide in rats (50 mg/kg × 10 days) decreased GSH content in the brain but GST activity was inhibited only by acrylamide and not by bis-acrylamide. Single or repeated injections of acrylamide but not of bis-acrylamide increased brain dopamine receptors (3H spiroperidol binding) in a concentration-dependent manner. [Description provided by NIOSH]
• Subjects:
  Animals Nervous System Occupational Health Safety
• Keywords:
  Acrylamide Animal Studies Author Keywords: Acrylamide Axonopathy Bis-acrylamide Brain Function Dopamine Receptor Dose Response Glutathione Glutathione-S-transferase Neuropathy Neurotoxic Effects Neurotoxicity Neurotoxin Neurotoxins

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• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  319-323
• Volume:
  371
• Issue:
  2
• NIOSHTIC Number:
  nn:20060318
• Citation:
  Brain Res 1986 Apr; 371(2):319-323
• Contact Point Address:
  M.I. Sabri, Institute of Neurotoxicology, Departments of Neurology and Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, U.S.A.
• CAS Registry Number:
  Acrylamide (CAS RN 79-06-1) ; Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  1986
• Performing Organization:
  Yeshiva University, New York, New York
• Peer Reviewed:
  True
• Start Date:
  19800501
• Source Full Name:
  Brain Research
• End Date:
  19890430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c87edc6270d03ef4d26ff6de6217509b50770c475bfb2e43dc79f38a29fee8f4d500c0123f2bdb93b07e03e348fe8ee49a03da39b1b5b20017731764d826c1f9
• Download URL:
  https://stacks.cdc.gov/view/cdc/224260/cdc_224260_DS1.pdf
• File Type:
  [PDF - 395.72 KB ]