Using Liquid Chromatography Mass Spectrometry (LC-MS) to Assess the Effect of Age, High-Fat Diet, and Rat Strain on the Liver Metabolome

Details

• Personal Author:
  Antonini JM ; Boyce G ; Erdely A ; Kashon M ; Kodali V ; Meighan T ; Roberts, Jennifer R. ; Shoeb M
• Description:
  The goal of this study was to use liquid chromatography mass spectrometry to assess metabolic changes of two different diets in three distinct rat strains. Sprague-Dawley, Fischer 344, and Brown-Norway male rats were maintained on a high-fat, or regular diet for 24 weeks. Liver tissue was collected at 4, 12, and 24 weeks to assess global small molecule metabolite changes using high resolution accurate mass spectrometry coupled to ultra-high-performance liquid chromatography. The results of the global metabolomics analysis revealed significant changes based on both age and diet within all three strains. Principal component analysis revealed that the influence of diet caused a greater variation in significantly changing metabolites than that of age for the Brown Norway and Fisher 344 strains, whereas diet had the greatest influence in the Sprague Dawley strain only at the 4-week time point. As expected, metabolites involved in lipid metabolism were changed in the animals maintained on a high fat diet compared to the regular diet. There were also significant changes observed in the concentration of Tri carboxylic acid cycle intermediates that were extracted from the liver of all three strains based on diet. The results of this study showed that a high fat diet caused significant liver and metabolic changes compared to a regular diet in multiple rat strains. The inbred Fisher 344 and Brown Norway rats were more metabolically sensitive to the diet changes than outbred Sprague Dawley strain. The study also showed that age, as was the case for Sprague Dawley, is an important variable to consider when assessing metabolic changes. [Description provided by NIOSH]
• Subjects:
  Acids Age Factors Diet Energy Metabolism Enzymes Laboratories Lipids Occupational Health Safety
• Keywords:
  Age Factors Amino Acids Carbohydrates Fats Laboratory Animals Laboratory Testing Liquid Chromatography Liver Liver Function Liver Tissue Mass Spectrometry Metabolic Effects Metabolic Study Metabolism Metabolites Proteins

  More +
• ISSN:
  1932-6203
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  15
• Issue:
  7
• NIOSHTIC Number:
  nn:20060288
• Citation:
  PLoS One 2020 Jul; 15(7):e0235338
• Contact Point Address:
  Greg Boyce, National Institute for Occupational Safety and Health, Morgantown, West Virginia, United States of America
• Email:
  omu0@cdc.gov
• CAS Registry Number:
  2-Oxoglutaric acid (CAS RN 328-50-7) ; Citric acid (CAS RN 77-92-9) ; Fumaric acid (CAS RN 110-17-8) ; Lactic acid (CAS RN 50-21-5) ; Malic acid (CAS RN 6915-15-7) ; Pyruvic acid (CAS RN 127-17-3) ; Succinic acid (CAS RN 110-15-6)
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  PLoS One
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:beac94308b5b1c39389e5517e52aefe87962b711fa22fbcec84fd6bf6aab7519ea05eb16efcd4d6501047e6fd858b2c788684b5981bf5a550676abdf6067ade5
• Download URL:
  https://stacks.cdc.gov/view/cdc/224238/cdc_224238_DS1.pdf
• File Type:
  [PDF - 1.29 MB ]