A Physico-Chemical Screening Test for Chemical Carcinogens: The Ke Test

Details

• Personal Author:
  Bakale G ; McCreary RD
• Description:
  A pulse-conductivity technique was used to measure the rate at which excess electrons in liquid cyclohexane attach to carcinogens and non-carcinogens in order to determine if the electron attachment rate constant, ke , could be used to screen potential carcinogens. The ke s of 114 chemicals are reported; these chemicals are among 182 that had previously been tested in a validation study of several short-term carcinogen-screening bioassays. The remaining 68 chemicals for which ke s were not measured include chemicals that were unavailable, were not sufficiently stable or soluble in cyclohexane, or did not have a well-defined mol. wt. For the 114 chemicals that were tested, 35 are carcinogens, 50 are putative non-carcinogens and 29 have not been adequately tested or yielded equivocal responses in animal-test studies. Diffusion-controlled ke s were measured for 27 of the 35 carcinogens tested whereas the ke s of 45 of the 50 non-carcinogens were less than diffusion controlled ke From these results, several measures of the predictive performance of using a diffusion-controlled ke to indicate a positive response to a carcinogen were calculated and compared with the Ames-test predictiveness in screening the same chemicals. The predictive criteria calculated were sensitivity, specificity, accuracy and predictive value, all of which were greater for the ke test than for the Ames test. Comparisons of the chemicals that yielded false-negative responses in the ke and Ames tests indicate a high degree of independence between the two which implies that the tests could be efficaciously used in a battery of short-term tests. Rationales are offered concerning the observed ke -carcinogencity correlation and the apparent lack of the need for procarcinogens to be metabolically activated to yield a positive ke response. [Description provided by NIOSH]
• Subjects:
  Biological Assay Carcinogens Chemistry, Analytic Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Methods Bioassays Chemicals Screening Methods
• ISSN:
  0143-3334
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  253-264
• Volume:
  8
• Issue:
  2
• NIOSHTIC Number:
  nn:20060251
• Citation:
  Carcinogenesis 1987 Feb; 8(2):253-264
• Federal Fiscal Year:
  1987
• Performing Organization:
  Case Western Reserve University, Cleveland, Ohio
• Peer Reviewed:
  True
• Start Date:
  19820801
• Source Full Name:
  Carcinogenesis
• End Date:
  19900630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c3cfe2c1ea1fd5339f5e1410dd3bd10ba48a0988171cf975d231de5844bb1d276d244ee65c5030c805719228470e80158a12b0fda008c28b615f70aaa13e23b5
• Download URL:
  https://stacks.cdc.gov/view/cdc/224209/cdc_224209_DS1.pdf
• File Type:
  [PDF - 663.02 KB ]