Ozone-Induced Alterations in Macrophage Interactions with Interferon-y

Details

• Personal Author:
  Cohen MD ; McManus T ; Schlesinger RB ; Zelikoff JT
• Description:
  Exposure to ozone is known to induce alterations in host resistance to bacterial pathogens and in the general function of lung macrophages. The priming of macrophages (M+) by immune system cytokines such as interferon-r (IFNy), IFNa, and tumor necrosis factor-a (lNFa) at the earliest stages of the immune response is crucial to both Mphi antimicrobial activity and to the subsequent activation of other cells such as lymphocytes and neutrophils. Cultured mouse macrophage-like WEHI-3 cells were exposed in vitro to atmospheres of 1.0 or 0.4 ppm ozone(O3), or air, for periods of 4-6 hr. The Mphi, were then assessed for their IFNy binding capacity and for the levels of IFNy-induced responses, i.e. reactive oxygen metabolite formation and enhanced phagocytic activity. The Mphi exposed to 1 ppm O3 had 60-65% decreases in radio labelled-IFNy binding as compared with air-treated cells; 0.4 ppm O3 treatment had no significant effect on binding. When the 1 ppm O3-treated cells were incubated with IFNy (100 U/ml) for 48 hr, cells produced 79% less superoxide anion radical as compared with IFNy-stimulated control cells; similar changes in hydrogen peroxide formation were also observed. While control WEHI-3 cells treated with IFNy displayed increases in their phagocytic activity (increased 35-40% compared with non-stimulated controls), no increases in activity in IFN-stimulated 1 ppm and 0.4 ppm O3-treated Mphi were observed; phagocytic activity actually decreased by 23 and 3%, respectively. These results demonstrate that in vitro exposure to ozone can modulate Mphi functions by affecting their interactions with a major early stage immunopotentiating agent, interferon-y. [Description provided by NIOSH]
• Subjects:
  Blood Immune System Lung Macrophages Occupational Health Ozone Respiratory System Safety
• Keywords:
  Cytokines Immune System Immunological Tests Lung Function
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  1
• NIOSHTIC Number:
  nn:20060168
• Citation:
  Toxicologist 1993 Mar; 13(1):421
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  1993
• Performing Organization:
  Mount Sinai School of Medicine, New York
• Peer Reviewed:
  False
• Start Date:
  19920701
• Source Full Name:
  The Toxicologist. Society of Toxicology 32nd Annual Meeting, March 14-18, 1993, New Orleans, Louisiana
• End Date:
  19970630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a6881c7a7cb30e64817c2d12a0e811b2568cc0fed973e57d150795ffd6dd5be0b5cd9ecfa56e55d8a6565e2b2d6223ced089f1d0338524698dfd029c8650138d
• Download URL:
  https://stacks.cdc.gov/view/cdc/224162/cdc_224162_DS1.pdf
• File Type:
  [PDF - 263.03 KB ]