Inflammatory Responses Following O3 Exposure: A Comparison of Sprague-Dawley Rats from Different Facilities of a Single Breeder

Details

• Personal Author:
  Gunnison AF ; Nadziejko C ; Weideman PA
• Description:
  Ozone (O3) has been shown to elicit a pulmonary inflammatory response, including the infiltration of neutrophils and extravasation of proteins into the alveolar spaces. Differences between strains of rats have been reported for inflammatory responses to acute O3 exposure. This study examined the inflammatory responses following O3 exposure of rats from different facilities of the same supplier. Adult, female Sprague-Dawley CD VAF rats were obtained from the Kingston, NY (KNY) and Raleigh, NC (RNC) facilities of Charles River Laboratories. Rats were exposed to 0, 0.3, 0.5 and 1.0 ppm O3 for 6 hours. The rats were killed 18 hrs after exposure and the lungs lavaged. Bronchoalveolar lavage fluid (BALF) was analyzed for total protein and differential cell counts. The rats from the KNY facility showed a greater increase in BALF protein and percent neutrophils than rats from the RNC facility at all 03 concentrations. Additional groups of rats from each facility were killed immediately upon receipt at the NYU facility and lungs were fixed for histopathological examination. The lungs from the KNY rats showed increased perivascular cuffing, i.e., presence of inflammatory cells around the blood vessels, relative to the RNC animals. Thus, a supply of rats that exhibited evidence of subclinical pulmonary histological changes (perivascular cuffing} was also more responsive to ozone-induced pulmonary inflammation compared to a supply of rats of the same strain that had a lesser degree of perivascular cuffing. These observations underscore the importance of colony variability in the selection of rats used in toxicologic investigations. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Lung Occupational Health Ozone Respiratory System Safety Toxicology
• Keywords:
  Animal Studies Exposure Assessment Immune Reaction Immune System Lung Tissue Pulmonary Effects
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  89
• Volume:
  14
• Issue:
  1
• NIOSHTIC Number:
  nn:20060144
• Citation:
  Toxicologist 1994 Mar; 14(1):89
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Mount Sinai School of Medicine, New York
• Peer Reviewed:
  False
• Start Date:
  19920701
• Source Full Name:
  The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
• End Date:
  19970630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1320c094b45348efb4fb09e10d169b50399f6b7575b8e808baea3683e03fcbf3feb90b4e268e0a376a987428d709e3f57718e1273e5e172098a5dc8e7ea268c8
• Download URL:
  https://stacks.cdc.gov/view/cdc/224147/cdc_224147_DS1.pdf
• File Type:
  [PDF - 270.78 KB ]