Inactivation of the Multi-Drug-Resistant Pathogen Candida auris Using Ultraviolet Germicidal Irradiation

Details

• Personal Author:
  Green, Brett J. ; Lemons, Angela R. ; Lindsley, William G. ; Martin, Stephen B. Jr. ; McClelland, Tia L.
• Description:
  Background: Candida auris, a multi-drug-resistant fungal pathogen, has become an emerging threat in healthcare settings around the world. Reliable disinfection protocols specifically designed to inactivate C. auris are essential, as many chemical disinfectants commonly used in healthcare settings have been shown to have variable efficacy at inactivating C. auris. Aim: Ultraviolet germicidal irradiation (UVGI) was investigated as a method to inactivate clinically relevant strains of C. auris. Methods: Ten C. auris and two C. albicans isolates were exposed to ultraviolet (UV) energy to determine the UV dose required to inactivate each isolate. Using a UV reactor, each isolate (106 cells/mL) was exposed to 11 UV doses ranging from 10 to 150 mJ/cm2 and then cultured to assess cell viability. Findings: An exponential decay model was applied to each dose-response curve to determine inactivation rate constants for each isolate, which ranged from 0.108 to 0.176 cm2/mJ for C. auris and from 0.239 to 0.292 cm2/mJ for C. albicans. As the model of exponential decay did not accurately estimate the dose beyond 99.9% inactivation, a logistic regression model was applied to better estimate the doses required for 99.999% inactivation. Using this model, significantly greater UV energy was required to inactivate C. auris (103-192 mJ/cm2) compared with C. albicans (78-80 mJ/cm2). Conclusion: UVGI is a feasible approach for inactivating C. auris, although variable susceptibility among isolates must be taken into account. This dose-response data is critical for recommending UVGI dosing strategies to be tested in healthcare settings. [Description provided by NIOSH]
• Subjects:
  Manufacturing And Industrial Facilities Mining Occupational Health Safety Staff Development
• Keywords:
  Author Keywords: Candida Auris Cell Cultures Cellular Reactions Disinfectants Disinfection Dose Response Drug Resistance Emerging Infectious Diseases Emerging Pathogen Fungal Infections Fungi Health Care Health Care Facilities Infectious Agents Infectious Diseases Irradiation MDR MDROs Medical Facilities Multidrug Resistant Multidrug Resistant Organisms Pathogens Ultraviolet Germicidal Irradiation Ultraviolet Inactivation Ultraviolet Radiation

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• ISSN:
  0195-6701
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  495-501
• Volume:
  105
• Issue:
  3
• NIOSHTIC Number:
  nn:20060124
• Citation:
  J Hosp Infect 2020 Jul; 105(3):495-501
• Contact Point Address:
  A. R. Lemons, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  wrw0@cdc.gov
• Federal Fiscal Year:
  2020
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Hospital Infection
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b2dca11a62760af9d04c6d3b1b4e4dd5d8808a1ee70215723a0bd5b699d91336a69db67f4e15963422c17fd69eb29534d555c7be563c11fd8a5909d351798263
• Download URL:
  https://stacks.cdc.gov/view/cdc/224134/cdc_224134_DS1.pdf
• File Type:
  [PDF - 325.89 KB ]